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Author (up) Casella, T.M.; Eparvier, V.; Mandavid, H.; Bendelac, A.; Odonne, G.; Dayan, L.; Duplais, C.; Espindola, L.S.; Stien, D. url  openurl
  Title Antimicrobial and cytotoxic secondary metabolites from tropical leaf endophytes: Isolation of antibacterial agent pyrrocidine C from Lewia infectoria SNB-GTC2402 Type Journal Article
  Year 2013 Publication Phytochemistry Abbreviated Journal Phytochemistry  
  Volume 96 Issue Pages 370-377  
  Keywords Antimicrobials; Cytotoxic metabolites; Functional chemodiversity; Leaf endophytes; Lewia; Pyrrocidine C  
  Abstract Because of the symbiotic nature of endophytes, this survey aims to investigate the probability of discovering antibacterial, antifungal and cytotoxic activities in leaf endophytic microbes. We isolated 138 cultivable microbes (121 fungi, 3 bacteria and 14 unidentified or unknown microbes) from 24 plant species, a significant relative proportion of which exhibited antifungal and cytotoxic potential against Candida albicans ATCC 10213 and the human cell lines KB (uterine cervical carcinoma), MDA-MB-435 (melanoma), and MRC5 (normal human lung fibroblasts). Three active fungal extracts were fractionated, resulting in the isolation of eight compounds. Seven had been described in the literature including the following: acremonisol A, semicochliodinol A, cochliodinol, griseofulvin, pyrenocin A, novae zelandin A and alterperylenol. A previously unreported compound named pyrrocidine C was isolated from Lewia infectoria SNB-GTC2402 and identified by spectroscopic analysis. As in pyrrocidines A and B, this compound is a cis-substituted decahydrofluorene with a quaternary carbon at C-5 and opposite stereochemistry at C-8 corresponding to C-6 of pyrrocidines A and B.© 2013 Elsevier Ltd. All rights reserved.  
  Address CNRS Guyane, USR 3456, 2 Avenue Gustave Charlery, 97300 Cayenne, France  
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  Series Volume Series Issue Edition  
  ISSN 00319422 (Issn) ISBN Medium  
  Area Expedition Conference  
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