| Record |
Author  |
Fu, T.; Houel, E.; Amusant, N.; Touboul, D.; Genta-Jouve, G.; Della-Negra, S.; Fisher, G.L.; Brunelle, A.; Duplais, C. |
| Title |
Biosynthetic investigation of γ-lactones in Sextonia rubra wood using in situ TOF-SIMS MS/MS imaging to localize and characterize biosynthetic intermediates |
Type |
Journal Article |
| Year |
2019 |
Publication |
Scientific Reports |
Abbreviated Journal |
Sci. Rep. |
| Volume |
9 |
Issue |
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Pages |
1928 |
| Keywords |
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| Abstract |
Molecular analysis by parallel tandem mass spectrometry (MS/MS) imaging contributes to the in situ characterization of biosynthetic intermediates which is crucial for deciphering the metabolic pathways in living organisms. We report the first use of TOF-SIMS MS/MS imaging for the cellular localization and characterization of biosynthetic intermediates of bioactive γ-lactones rubrynolide and rubrenolide in the Amazonian tree Sextonia rubra (Lauraceae). Five γ-lactones, including previously reported rubrynolide and rubrenolide, were isolated using a conventional approach and their structural characterization and localization at a lateral resolution of ~400 nm was later achieved using TOF-SIMS MS/MS imaging analysis. 2D/3D MS imaging at subcellular level reveals that putative biosynthetic γ-lactones intermediates are localized in the same cell types (ray parenchyma cells and oil cells) as rubrynolide and rubrenolide. Consequently, a revised metabolic pathway of rubrynolide was proposed, which involves the reaction between 2-hydroxysuccinic acid and 3-oxotetradecanoic acid, contrary to previous studies suggesting a single polyketide precursor. Our results provide insights into plant metabolite production in wood tissues and, overall, demonstrate that combining high spatial resolution TOF-SIMS imaging and MS/MS structural characterization offers new opportunities for studying molecular and cellular biochemistry in plants. © 2019, The Author(s). |
| Address |
Physical Electronics, Chanhassen, MN 55317, United States |
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| Publisher |
Nature Publishing Group |
Place of Publication |
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Edition |
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| ISSN |
20452322 (Issn) |
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| Call Number |
EcoFoG @ webmaster @ |
Serial |
866 |
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