| Record |
| Author |
Zinger, L.; Chave, J.; Coissac, E.; Iribar, A.; Louisanna, E.; Manzi, S.; Schilling, V.; Schimann, H.; Sommeria-Klein, G.; Taberlet, P. |
Title  |
Extracellular DNA extraction is a fast, cheap and reliable alternative for multi-taxa surveys based on soil DNA |
Type |
Journal Article |
| Year |
2016 |
Publication |
Soil Biology and Biochemistry |
Abbreviated Journal |
Soil Biology and Biochemistry |
| Volume |
96 |
Issue |
|
Pages |
16-19 |
| Keywords |
DNA extraction protocol; DNA metabarcoding; Multi-taxa biodiversity; Tropical forest |
| Abstract |
DNA metabarcoding on soil samples is increasingly used for large-scale and multi-taxa biodiversity studies. However, DNA extraction may be a major bottleneck for such wide uses. It should be cost/time effective and allow dealing with large sample volumes so as to maximise the representativeness of both micro- and macro-organisms diversity. Here, we compared the performances of a fast and cheap extracellular DNA extraction protocol with a total DNA extraction method in retrieving bacterial, eukaryotic and plant diversity from tropical soil samples of ca. 10 g. The total DNA extraction protocol yielded more high-quality DNA. Yet, the extracellular DNA protocol provided similar diversity assessments although it presented some differences in clades relative abundance and undersampling biases. We argue that extracellular DNA is a good compromise between cost, labor, and accuracy for high-throughput DNA metabarcoding studies of soil biodiversity. © 2016 Elsevier Ltd. |
| Address |
INRA UMR ECOFOG, Kourou, French Guiana |
| Corporate Author |
|
Thesis |
|
| Publisher |
|
Place of Publication |
|
Editor |
|
| Language |
|
Summary Language |
|
Original Title |
|
| Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
| Series Volume |
|
Series Issue |
|
Edition |
|
| ISSN |
|
ISBN |
|
Medium |
|
| Area |
|
Expedition |
|
Conference |
|
| Notes |
Export Date: 17 February 2016 |
Approved |
no |
| Call Number |
EcoFoG @ webmaster @ |
Serial |
663 |
| Permanent link to this record |
