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Author Gonzalez, M.A.; Baraloto, C.; Engel, J.; Mori, S.A.; Petronelli, P.; Riera, B.; Roger, A.; Thebaud, C.; Chave, J. pdf  openurl
  Title Identification of Amazonian Trees with DNA Barcodes Type Journal Article
  Year 2009 Publication PLoS One Abbreviated Journal PLoS One  
  Volume 4 Issue 10 Pages e7483  
  Abstract Background: Large-scale plant diversity inventories are critical to develop informed conservation strategies. However, the workload required for classic taxonomic surveys remains high and is particularly problematic for megadiverse tropical forests. Methodology/Principal Findings: Based on a comprehensive census of all trees in two hectares of a tropical forest in French Guiana, we examined whether plant DNA barcoding could contribute to increasing the quality and the pace of tropical plant biodiversity surveys. Of the eight plant DNA markers we tested (rbcLa, rpoC1, rpoB, matK, ycf5, trnL, psbA-trnH, ITS), matK and ITS had a low rate of sequencing success. More critically, none of the plastid markers achieved a rate of correct plant identification greater than 70%, either alone or combined. The performance of all barcoding markers was noticeably low in few species-rich clades, such as the Laureae, and the Sapotaceae. A field test of the approach enabled us to detect 130 molecular operational taxonomic units in a sample of 252 juvenile trees. Including molecular markers increased the identification rate of juveniles from 72% (morphology alone) to 96% (morphology and molecular) of the individuals assigned to a known tree taxon. Conclusion/Significance: We conclude that while DNA barcoding is an invaluable tool for detecting errors in identifications and for identifying plants at juvenile stages, its limited ability to identify collections will constrain the practical implementation of DNA-based tropical plant biodiversity programs.  
  Corporate Author Thesis  
  Publisher PUBLIC LIBRARY SCIENCE Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1932-6203 ISBN (up) Medium  
  Area Expedition Conference  
  Notes ISI:000270880700009 Approved no  
  Call Number EcoFoG @ eric.marcon @ Serial 189  
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