|
|
Touchard, A., Aili, S. R., Téné, N., Barassé, V., Klopp, C., Dejean, A., et al. (2020). Venom Peptide Repertoire of the European Myrmicine Ant Manica rubida: Identification of Insecticidal Toxins. J. Proteome Res., 19(4), 1800–1811.
Abstract: Using an integrated transcriptomic and proteomic approach, we characterized the venom peptidome of the European red ant, Manica rubida. We identified 13 “myrmicitoxins” that share sequence similarities with previously identified ant venom peptides, one of them being identified as an EGF-like toxin likely resulting from a threonine residue modified by O-fucosylation. Furthermore, we conducted insecticidal assays of reversed-phase HPLC venom fractions on the blowfly Lucilia caesar, permitting us to identify six myrmicitoxins (i.e., U3-, U10-, U13-, U20-MYRTX-Mri1a, U10-MYRTX-Mri1b, and U10-MYRTX-Mri1c) with an insecticidal activity. Chemically synthesized U10-MYRTX-Mri1a, -Mri1b, -Mri1c, and U20-MYRTX-Mri1a irreversibly paralyzed blowflies at the highest doses tested (30-125 nmol·g-1). U13-MYRTX-Mri1a, the most potent neurotoxic peptide at 1 h, had reversible effects after 24 h (150 nmol·g-1). Finally, U3-MYRTX-Mri1a has no insecticidal activity, even at up to 55 nmol·g-1. Thus, M. rubida employs a paralytic venom rich in linear insecticidal peptides, which likely act by disrupting cell membranes.
Keywords: glycosylated toxin; peptidome; polycationic α-helix; predation; pyroglutamate; reversible neurotoxicity
|
|
|
|
Foucaud, J., Orivel, J., Fournier, D., Delabie, J. H. C., Loiseau, A., Le Breton, J., et al. (2009). Reproductive system, social organization, human disturbance and ecological dominance in native populations of the little fire ant, Wasmannia auropunctata. Mol. Ecol., 18(24), 5059–5073.
Abstract: The invasive ant species Wasmannia auropunctata displays both ecologically dominant and non-dominant populations within its native range. Three factors could theoretically explain the ecological dominance of some native populations of W. auropunctata: (i) its clonal reproductive system, through demographic and/or adaptive advantages; (ii) its unicolonial social organization, through lower intraspecific and efficient interspecific competition; (iii) the human disturbance of its native range, through the modification of biotic and abiotic environmental conditions. We used microsatellite markers and behavioural tests to uncover the reproductive modes and social organization of dominant and non-dominant native populations in natural and human-modified habitats. Microsatellite and mtDNA data indicated that dominant and non-dominant native populations (supercolonies as determined by aggression tests) of W. auropunctata did not belong to different evolutionary units. We found that the reproductive system and the social organization are neither necessary nor sufficient to explain W. auropunctata ecological dominance. Dominance rather seems to be set off by unknown ecological factors altered by human activities, as all dominant populations were recorded in human-modified habitats. The clonal reproductive system found in some populations of W. auropunctata may however indirectly contribute to its ecological dominance by allowing the species to expand its environmental niche, through the fixation over time of specific combinations of divergent male and female genotypes. Unicoloniality may rather promote the range expansion of already dominant populations than actually trigger ecological dominance. The W. auropunctata model illustrates the strong impact of human disturbance on species' ecological features and the adaptive potential of clonal reproductive systems.
Keywords: biological invasion; clonality; human disturbance; parthenogenesis; social organization; Wasmannia auropunctata
|
|
|
|
Cohen, M. C. L., Behling, H., Lara, R. J., Smith, C. B., Matos, H. R. S., & Vedel, V. (2009). Impact of sea-level and climatic changes on the Amazon coastal wetlands during the late Holocene. Veg. Hist. Archaeobot., 18(6), 425–439.
Abstract: Wetland dynamics in northern Brazil during the Holocene were studied by pollen analysis and AMS radiocarbon dating of three cores. Near the Amazon mouth region, covered mainly by primary Amazon coastal forest and herbaceous vegetation, the pollen record indicates the dominance of mangroves between 4800 and 1100 cal yr B.P. A contraction of the mangrove area and an expansion of herbaceous and fern vegetation occurred between 1100 and 750 cal yr B.P. The period between 750 and 200 cal yr B.P. is characterized by an expansion of mangrove and a decrease in herbaceous and fern vegetation. This trend continued until the present. On Atalaia Island, the sediment core indicates a period with poor pollen preservation between 830 and 630 cal yr B.P. Between 630 and 330 cal yr B.P., mangroves expanded. Later, up to 45 cal yr B.P., the mangrove area decreased and the herbaceous vegetation expanded. During the last hundred years, the relative sea-level rise most probably favored the mangrove expansion as far as the topographically highest sector on this island, while the herbaceous vegetation decreased. The pollen data from Agua Preta Lake indicate dry conditions, as reflected by the poor pollen preservation between 390 and 240 cal yr B.P. Between 240 and 60 cal yr B.P., restinga and Amazon coastal forest with palms dominated this region. For the last 120 years, the record indicates an expansion of the mangrove area. However, recent confinement of mangrove development to the topographically highest area, and the loss of mangrove areas on the lowest surfaces have led to a net loss of mangrove coverage during the last decades.
Keywords: Mangrove; Marajo Island; Pollen analysis; Sea-level
|
|
|
|
Salhi, L., Nait-Rabah, O., Deyrat, C., & Roos, C. (2013). Numerical Modeling of Single Helical Pile Behavior under Compressive Loading in Sand. Electron. J. Geotech. Eng., 18(Bundle T), 4119–4338.
Abstract: The present research deals with helical piles behavior in cohesionless soil through finite element modeling. An approach of modeling of the screw-pile geometry has been proposed through the Finite Element Analysis (FEA) computer program Plaxis. The numerical results are compared with measurements from large scale test and the bearing capacity has been estimated using both cylindrical and individual bearing model. Moreover, different failure criterions have been applied to estimate the ultimate capacity. The effect of spacing ratio (S/Dh) on the screw-pile behavior has been further studied. It has found that results from the model fit the field results. Through the study of the load transfer mechanism, the transition from cylindrical shear to individual plate behavior occurs at a value of spacing ratio (1.5 to 2).
Keywords: helical pile; finite element method; failure mechanisms; sand
|
|
|
|
Sist, P., Rutishauser, E., Peña-Claros, M., Shenkin, A., Herault, B., Blanc, L., et al. (2015). The Tropical managed forests Observatory: A research network addressing the future of tropical logged forests. Appl. Veg. Sci., 18(1), 171–174.
Abstract: While attention on logging in the tropics has been increasing, studies on the long-term effects of silviculture on forest dynamics and ecology remain scare and spatially limited. Indeed, most of our knowledge on tropical forests arises from studies carried out in undisturbed tropical forests. This bias is problematic given that logged and disturbed tropical forests are now covering a larger area than the so-called primary forests. A new network of permanent sample plots in logged forests, the Tropical managed Forests Observatory (TmFO), aims to fill this gap by providing unprecedented opportunities to examine long-term data on the resilience of logged tropical forests at regional and global scales. TmFO currently includes 24 experimental sites distributed across three tropical regions, with a total of 490 permanent plots and 921 ha of forest inventories. To improve our knowledge of the resilience of tropical logged forests, 20 research institutes are now collaborating on studies on the effects of logging on forest structure, productivity, biodiversity and carbon fluxes at large spatial and temporal scales. These studies are carried out in the Tropical managed Forests Observatory (TmFO), an international network including 24 sites and 490 permanent sample plots across South America, Africa and South East Asia.
Keywords: Biodiversity; Carbon cycle; Climate change; Ecosystem resilience; Logging; Silviculture; Tropical forests; Tropical managed forests Observatory
|
|
|
|
Siefert, A., Violle, C., Chalmandrier, L., Albert, C. H., Taudiere, A., Fajardo, A., et al. (2015). A global meta-analysis of the relative extent of intraspecific trait variation in plant communities. Ecology Letters, 18(12), 1406–1419.
Abstract: Recent studies have shown that accounting for intraspecific trait variation (ITV) may better address major questions in community ecology. However, a general picture of the relative extent of ITV compared to interspecific trait variation in plant communities is still missing. Here, we conducted a meta-analysis of the relative extent of ITV within and among plant communities worldwide, using a data set encompassing 629 communities (plots) and 36 functional traits. Overall, ITV accounted for 25% of the total trait variation within communities and 32% of the total trait variation among communities on average. The relative extent of ITV tended to be greater for whole-plant (e.g. plant height) vs. organ-level traits and for leaf chemical (e.g. leaf N and P concentration) vs. leaf morphological (e.g. leaf area and thickness) traits. The relative amount of ITV decreased with increasing species richness and spatial extent, but did not vary with plant growth form or climate. These results highlight global patterns in the relative importance of ITV in plant communities, providing practical guidelines for when researchers should include ITV in trait-based community and ecosystem studies. © 2015 John Wiley & Sons Ltd/CNRS.
Keywords: Community ecology; Functional diversity; Interspecific variation; Intraspecific variability; Leaf trait; Plant functional trait; Trait-based ecology
|
|
|
|
Duplais, C., & Estevez, Y. (2017). Tandem Biocatalysis Unlocks the Challenging de Novo Production of Plant Natural Products. ChemBioChem, 18(22), 2192–2195.
Abstract: Intimate partnership: Knowledge of the biocatalytic cascades in different cellular compartments is limited, but deciphering these systems in nature can be used to inspire synthetic strategies. Two studies report new insights into the biosynthesis of alkaloids and sesterterpenoids in plants. This highlight presents these novel biotransformations to illustrate how tandem biocatalysis can impact the future of natural product production.
Keywords: alkaloids; biosynthesis; enzyme catalysis; protein engineering; terpenes
|
|
|
|
Herault, B., & Thoen, D. (2008). Diversity of plant assemblages in isolated depressional wetlands from Central-Western Europe. Biodivers. Conserv., 17(9), 2169–2183.
Abstract: A closed depression is defined as a geomorphologic element where a sediment depository is encircled by hillslopes. Despite the fact that closed depressions are often the only stagnant water points in many European landscapes, few ecological researches on their plant assemblages have been done. The main goal of this study was to give first results of the environmental factors responsible for the vegetation composition, richness and rarity in the closed depressions of the Lorraine biogeographical district (Belgium, France and grand-duche de Luxembourg). We surveyed for plant presence 85 forest and 77 grassland closed depressions. For each site, wetland area, local environmental factors and regional connectivities registered. For each species, the Ellenberg values were compiled. To investigate the main source of variation in species composition and in species richness (including richness in rare species), Non-metric Multidimensional Scaling analyses and Generalized Linear Models were respectively used. Species pools in forest (forest and preforest species) and in grassland (bog plants, pioneers, helophytes) were quite different. In both landscapes, a gradient from plants typical of basic high-productive soils to plants typical of acid low-productive soils reflects a shared successional gradient. The accumulation of organic matter allowed the establishment of Sphagnum spp., which slowly acidified the soil and thus acted as ecosystem engineers for the arrival of bog plants. Moreover, the species composition was additionally driven by the plant light tolerance in forests and by the plant water requirements in grasslands. Mechanisms of species accumulation (increase in species richness) were different in forests and in grasslands: respectively related to the plant light tolerance and to the wetland area. At the regional level, the averaged soil productivity was negatively related to the richness in rare species. Indeed, perennial highly-competitive plants such as Glyceria spp., Iris pseudacorus or Urtica dioica impeded the establishment of smaller and rarer species. At the habitat level, isolated closed depressions (due to either low connectivity or low grazing pressure) have more habitat rare species, giving evidences of dispersal limitation in plant assemblages of closed depressions.
Keywords: closed depressions; connectivity; fens; forest; grassland; habitat area; landscape matrix
|
|
|
|
Baraloto, C., Rabaud, S., Molto, Q., Blanc, L., Fortunel, C., Herault, B., et al. (2011). Disentangling stand and environmental correlates of aboveground biomass in Amazonian forests. Glob. Change Biol., 17(8), 2677–2688.
Abstract: Tropical forests contain an important proportion of the carbon stored in terrestrial vegetation, but estimated aboveground biomass (AGB) in tropical forests varies two-fold, with little consensus on the relative importance of climate, soil and forest structure in explaining spatial patterns. Here, we present analyses from a plot network designed to examine differences among contrasting forest habitats (terra firme, seasonally flooded, and white-sand forests) that span the gradient of climate and soil conditions of the Amazon basin. We installed 0.5-ha plots in 74 sites representing the three lowland forest habitats in both Loreto, Peru and French Guiana, and we integrated data describing climate, soil physical and chemical characteristics and stand variables, including local measures of wood specific gravity (WSG). We use a hierarchical model to separate the contributions of stand variables from climate and soil variables in explaining spatial variation in AGB. AGB differed among both habitats and regions, varying from 78 Mg ha(-1) in white-sand forest in Peru to 605 Mg ha(-1) in terra firme clay forest of French Guiana. Stand variables including tree size and basal area, and to a lesser extent WSG, were strong predictors of spatial variation in AGB. In contrast, soil and climate variables explained little overall variation in AGB, though they did co-vary to a limited extent with stand parameters that explained AGB. Our results suggest that positive feedbacks in forest structure and turnover control AGB in Amazonian forests, with richer soils (Peruvian terra firme and all seasonally flooded habitats) supporting smaller trees with lower wood density and moderate soils (French Guianan terra firme) supporting many larger trees with high wood density. The weak direct relationships we observed between soil and climate variables and AGB suggest that the most appropriate approaches to landscape scale modeling of AGB in the Amazon would be based on remote sensing methods to map stand structure.
Keywords: carbon stocks; climate; flooded forest; forest structure; French Guiana; Peru; REDD; soil properties; tropical rainforest; white-sand forest; wood specific gravity
|
|
|
|
Lang, G., & Marcon, E. (2013). Testing randomness of spatial point patterns with the Ripley statistic. ESAIM PS, 17, 767–788.
Abstract: Aggregation patterns are often visually detected in sets of location data. These clusters may be the result of interesting dynamics or the effect of pure randomness. We build an asymptotically Gaussian test for the hypothesis of randomness corresponding to a homogeneous Poisson point process. We first compute the exact first and second moment of the Ripley K-statistic under the homogeneous Poisson point process model. Then we prove the asymptotic normality of a vector of such statistics for different scales and compute its covariance matrix. From these results, we derive a test statistic that is chi-square distributed. By a Monte-Carlo study, we check that the test is numerically tractable even for large data sets and also correct when only a hundred of points are observed
Keywords: Central limit theorem, goodness-of-fit test, Höffding decomposition, null, point pattern, Poisson process, null
|
|
