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Author |
Zinger, L.; Chave, J.; Coissac, E.; Iribar, A.; Louisanna, E.; Manzi, S.; Schilling, V.; Schimann, H.; Sommeria-Klein, G.; Taberlet, P. |
Title |
Extracellular DNA extraction is a fast, cheap and reliable alternative for multi-taxa surveys based on soil DNA |
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Journal Article |
Year |
2016 |
Publication |
Soil Biology and Biochemistry |
Abbreviated Journal |
Soil Biology and Biochemistry |
Volume |
96 |
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Pages |
16-19 |
Keywords |
DNA extraction protocol; DNA metabarcoding; Multi-taxa biodiversity; Tropical forest |
Abstract |
DNA metabarcoding on soil samples is increasingly used for large-scale and multi-taxa biodiversity studies. However, DNA extraction may be a major bottleneck for such wide uses. It should be cost/time effective and allow dealing with large sample volumes so as to maximise the representativeness of both micro- and macro-organisms diversity. Here, we compared the performances of a fast and cheap extracellular DNA extraction protocol with a total DNA extraction method in retrieving bacterial, eukaryotic and plant diversity from tropical soil samples of ca. 10 g. The total DNA extraction protocol yielded more high-quality DNA. Yet, the extracellular DNA protocol provided similar diversity assessments although it presented some differences in clades relative abundance and undersampling biases. We argue that extracellular DNA is a good compromise between cost, labor, and accuracy for high-throughput DNA metabarcoding studies of soil biodiversity. © 2016 Elsevier Ltd. |
Address |
INRA UMR ECOFOG, Kourou, French Guiana |
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Export Date: 17 February 2016 |
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EcoFoG @ webmaster @ |
Serial |
663 |
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