| Records |
| Author |
Zinger, L.; Donald, J.; Brosse, S.; Gonzalez, M.A.; Iribar, A.; Leroy, C.; Murienne, J.; Orivel, J.; Schimann, H.; Taberlet, P.; Lopes, C.M. |
| Title |
Advances and prospects of environmental DNA in neotropical rainforests |
Type |
Journal Article |
| Year |
2020 |
Publication |
Advances in Ecological Research |
Abbreviated Journal |
Adv. Ecol. Res. |
| Volume |
62 |
Issue |
|
Pages  |
331-373 |
| Keywords |
Biomonitoring; Conservation biology; DNA metabarcoding; eDNA; Environmental genomics; Neotropics; Rainforests |
| Abstract |
The rainforests of the Neotropics shelter a vast diversity of plant, animal and microscopic species that provide critical ecosystem goods and services for both local and worldwide populations. These environments face a major crisis due to increased deforestation, pollution, and climate change, emphasizing the need for more effective conservation efforts. The adequate monitoring of these ecosystems has proven a complex and time consuming endeavour, which depends on ever dwindling taxonomic expertise. To date, many species remain undiscovered, let alone described, with otherwise limited information regarding known species population distributions and densities. Overcoming these knowledge shortfalls and practical limitations is becoming increasingly possible through techniques based on environmental DNA (eDNA), i.e., DNA that can be obtained from environmental samples (e.g. tissues, soil, sediment, water, etc.). When coupled with high-throughput sequencing, these techniques now enable realistic, cost-effective, and standardisable biodiversity assessments. This opens up enormous opportunities for advancing our understanding of complex and species-rich tropical communities, but also in facilitating large-scale biomonitoring programs in the neotropics. In this review, we provide a brief introduction to eDNA methods, and an overview of their current and potential uses in both terrestrial and aquatic ecosystems of neotropical rainforests. We also discuss the limits and challenges of these methods for our understanding and monitoring of biodiversity, as well as future research and applied perspectives of these techniques in neotropical rainforests, and beyond. © 2020 Elsevier Ltd |
| Address |
Departamento de Zoologia, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, SP, Brazil |
| Corporate Author |
|
Thesis |
|
| Publisher |
Academic Press Inc. |
Place of Publication |
|
Editor |
Dumbrell A.J.; Turner E.C.; Fayle T.M. |
| Language |
|
Summary Language |
|
Original Title |
|
| Series Editor |
|
Series Title |
|
Abbreviated Series Title |
Advances in Ecological Research |
| Series Volume |
62 |
Series Issue |
|
Edition |
|
| ISSN |
00652504 (Issn); 9780128211342 (Isbn) |
ISBN |
|
Medium |
|
| Area |
|
Expedition |
|
Conference |
|
| Notes |
|
Approved |
no |
| Call Number |
EcoFoG @ webmaster @ |
Serial |
995 |
| Permanent link to this record |
| |
|
| |
| Author |
Clair, B.; Ruelle, J.; Beauchene, J.; Prevost, M.F.; Fournier, M. |
| Title |
Tension wood and opposite wood in 21 tropical rain forest species 1. Occurrence and efficiency of the G-layer |
Type |
Journal Article |
| Year |
2006 |
Publication |
IAWA Journal |
Abbreviated Journal |
IAWA J. |
| Volume |
27 |
Issue |
3 |
Pages |
329-338 |
| Keywords |
gelatinous layer; G-layer; French Guyana; tropical rain forest; tension wood; wood anatomy |
| Abstract |
Wood samples were taken from the upper and lower sides of 21 naturally tilted trees from 18 families of angiosperms in the tropical rain forest in French Guyana. The measurement of growth stresses ensured that the two samples were taken from wood tissues in a different mechanical state: highly tensile stressed wood on the upper side, called tension wood, and lower tensile stressed wood on the lower side, called opposite wood. Eight species had tension wood fibres with a distinct gelatinous layer (G-layer). The distribution of gelatinous fibres varied from species to species. One of the species, Casearia javitensis (Flacourtiaceae), showed a peculiar multilayered secondary wall in its reaction wood. Comparison between the stress level and the occurrence of the G-layer indicates that the G-layer is not a key factor in the production of high tensile stressed wood. |
| Address |
UAG, INRA, ENGREF, CIRAD CNRS,ECOFOG,UMR Ecol Forets Guyane, F-97379 Kourou, Guyana, Email: clair@lmgc.univ-montp2.fr |
| Corporate Author |
|
Thesis |
|
| Publisher |
INT ASSOC WOOD ANATOMISTS |
Place of Publication |
|
Editor |
|
| Language |
|
Summary Language |
|
Original Title |
|
| Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
| Series Volume |
|
Series Issue |
|
Edition |
|
| ISSN |
0928-1541 |
ISBN |
|
Medium |
|
| Area |
|
Expedition |
|
Conference |
|
| Notes |
ISI:000240542400008 |
Approved |
no |
| Call Number |
EcoFoG @ eric.marcon @ |
Serial |
176 |
| Permanent link to this record |
| |
|
| |
| Author |
Chaves, C.L.; Blanc-Jolivet, C.; Sebbenn, A.M.; Mader, M.; Meyer-Sand, B.R.V.; Paredes-Villanueva, K.; Honorio Coronado, E.N.; Garcia-Davila, C.; Tysklind, N.; Troispoux, V.; Massot, M.; Degen, B. |
| Title |
Nuclear and chloroplastic SNP markers for genetic studies of timber origin for Hymenaea trees |
Type |
Journal Article |
| Year |
2019 |
Publication |
Conservation Genetics Resources |
Abbreviated Journal |
Conserv. Gen. Res. |
| Volume |
11 |
Issue |
3 |
Pages |
329-331 |
| Keywords |
DNA fingerprints; Geographical origin; MiSeq; RADSeq |
| Abstract |
We developed nuclear and chloroplastic single nucleotide polymorphism (SNP) and INDEL (insertion/deletion) markers using restriction associated DNA sequencing (RADSeq) and low coverage MiSeq genome sequencing to set up a genetic tracking method of the geographical origin of Hymenaea sp. From two initial sets of 358 and 32 loci used to genotype at least 94 individuals, a final set of 75 nSNPs, 50 cpSNPs and 6 INDELs identifying significant population structure was developed. © 2018, Springer Nature B.V. |
| Address |
Departamento de Fitotecnia, Faculdade de Engenharia de Ilha Solteira, Universidade Estadual Paulista, Ilha Solteira, SP, Brazil |
| Corporate Author |
|
Thesis |
|
| Publisher |
Springer Netherlands |
Place of Publication |
|
Editor |
|
| Language |
|
Summary Language |
|
Original Title |
|
| Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
| Series Volume |
|
Series Issue |
|
Edition |
|
| ISSN |
18777252 (Issn) |
ISBN |
|
Medium |
|
| Area |
|
Expedition |
|
Conference |
|
| Notes |
|
Approved |
no |
| Call Number |
EcoFoG @ webmaster @ |
Serial |
908 |
| Permanent link to this record |
| |
|
| |
| Author |
N'Guessan, A.E.; N'dja, J.K.; Yao, O.N.; Amani, B.H.K.; Gouli, R.G.Z.; Piponiot, C.; Zo-Bi, I.C.; Herault, B. |
| Title |
Drivers of biomass recovery in a secondary forested landscape of West Africa |
Type |
Journal Article |
| Year |
2019 |
Publication |
Forest Ecology and Management |
Abbreviated Journal |
|
| Volume |
433 |
Issue |
|
Pages |
325-331 |
| Keywords |
Biomass; Cultivation; Ecology; Recovery; Secondary recovery; Agricultural land; Bayesian frameworks; Diameter-at-breast heights; Forested landscapes; Neotropical forests; Old-growth forest; Physical environments; Secondary forests; Forestry; Dioscorea alata |
| Abstract |
The rapidly growing human population in West Africa has generated increasing demand for agricultural land and forest products. Consequently 90% of the original rainforest cover has now disappeared and the remainder is heavily fragmented and highly degraded. Although many studies have focused on carbon stocks and fluxes in intact African forests, little information exists on biomass recovery rates in secondary forests. We studied a chronosequence of 96 secondary and old-growth forest fragments (0.2 ha each) where 32.103 trees with Diameter at Breast Height > 2.5 cm have been censused. We modelled the biomass recovery trajectories in a time-explicit Bayesian framework and tested the effect on recovery rates of a large set of covariates related to the physical environment, plot history, and forest connectivity. Recovery rate trajectory is highly non-linear: recovery rates accelerated from 1 to 37 years, when biomass recovery reached 4.23 Mg /ha /yr, and decelerated afterwards. We predict that, on average, 10%, 25% and 50% of the old-growth forest biomass is respectively recovered 17, 30, and 51 years after abandonment. Recovery rates are strongly shaped by both the number of remnant trees (residuals of the former old-growth forest) and the previous crop cultivated before abandonment. The latter induced large differences in the time needed to recover 50% of an old-growth forest biomass: from 38 years for former Yam fields up to 86 years for former rice fields. Our results emphasize (i) the very slow recovery rates of West African forests, as compared to Neotropical forests (ii) the long-lasting impacts of past human activities and management choices on ecosystem biomass recovery in West African degraded forests. |
| Address |
|
| Corporate Author |
|
Thesis |
|
| Publisher |
Elsevier B.V. |
Place of Publication |
|
Editor |
|
| Language |
|
Summary Language |
|
Original Title |
|
| Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
| Series Volume |
|
Series Issue |
|
Edition |
|
| ISSN |
03781127 (Issn) |
ISBN |
|
Medium |
|
| Area |
|
Expedition |
|
Conference |
|
| Notes |
|
Approved |
no |
| Call Number |
EcoFoG @ webmaster @ |
Serial |
838 |
| Permanent link to this record |
| |
|
| |
| Author |
Dejean, A.; Labrière, N.; Touchard, A.; Petitclerc, F.; Roux, O. |
| Title |
Nesting habits shape feeding preferences and predatory behavior in an ant genus |
Type |
Journal Article |
| Year |
2014 |
Publication |
Naturwissenschaften |
Abbreviated Journal |
Naturwissenschaften |
| Volume |
101 |
Issue |
4 |
Pages |
323-330 |
| Keywords |
Ant genus Pseudomyrmex; Arboreal and ground nesting; Feeding preferences; Myrmecophytism; Predation |
| Abstract |
We tested if nesting habits influence ant feeding preferences and predatory behavior in the monophyletic genus Pseudomyrmex (Pseudomyrmecinae) which comprises terrestrial and arboreal species, and, among the latter, plant-ants which are obligate inhabitants of myrmecophytes (i.e., plants sheltering so-called plant-ants in hollow structures). A cafeteria experiment revealed that the diet of ground-nesting Pseudomyrmex consists mostly of prey and that of arboreal species consists mostly of sugary substances, whereas the plant-ants discarded all the food we provided. Workers forage solitarily, detecting prey from a distance thanks to their hypertrophied eyes. Approach is followed by antennal contact, seizure, and the manipulation of the prey to sting it under its thorax (next to the ventral nerve cord). Arboreal species were not more efficient at capturing prey than were ground-nesting species. A large worker size favors prey capture. Workers from ground- and arboreal-nesting species show several uncommon behavioral traits, each known in different ant genera from different subfamilies: leaping abilities, the use of surface tension strengths to transport liquids, short-range recruitment followed by conflicts between nestmates, the consumption of the prey's hemolymph, and the retrieval of entire prey or pieces of prey after having cut it up. Yet, we never noted group ambushing. We also confirmed that Pseudomyrmex plant-ants live in a kind of food autarky as they feed only on rewards produced by their host myrmecophyte, or on honeydew produced by the hemipterans they attend and possibly on the fungi they cultivate. © 2014 Springer-Verlag Berlin Heidelberg. |
| Address |
IRD, MIVEGEC (IRD 224-CNRS 5290-UM1-UM2), Équipe BEES, 911 avenue Agropolis, 34394 Montpellier Cedex 5, France |
| Corporate Author |
|
Thesis |
|
| Publisher |
Springer Verlag |
Place of Publication |
|
Editor |
|
| Language |
|
Summary Language |
|
Original Title |
|
| Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
| Series Volume |
|
Series Issue |
|
Edition |
|
| ISSN |
00281042 (Issn) |
ISBN |
|
Medium |
|
| Area |
|
Expedition |
|
Conference |
|
| Notes |
Export Date: 5 May 2014; Source: Scopus; Coden: Natwa; Language of Original Document: English; Correspondence Address: Dejean, A.; CNRS, Écologie des Forêts de Guyane (UMR-CNRS 8172), Campus Agronomique, BP 316, 97379 Kourou cedex, France; email: alain.dejean@wanadoo.fr |
Approved |
no |
| Call Number |
EcoFoG @ webmaster @ |
Serial |
539 |
| Permanent link to this record |
| |
|
| |
| Author |
Scotti, I.; Calvo-Vialettes, L.; Scotti-Saintagne, C.; Citterio, M.; Degen, B.; Bonal, D. |
| Title |
Genetic variation for growth, morphological, and physiological traits in a wild population of the Neotropical shade tolerant rainforest tree Sextonia rubra (Mez) van der Werff (Lauraceae) |
Type |
Journal Article |
| Year |
2010 |
Publication |
Tree Genetics & Genomes |
Abbreviated Journal |
Tree Genet. Genomes |
| Volume |
6 |
Issue |
2 |
Pages |
319-329 |
| Keywords |
Ecophysiological traits; Heritability; Guiana shield; Amazon; Ecological genetics |
| Abstract |
Quantitative genetic diversity is a fundamental component of the interaction between natural populations and their environment. In breeding programmes, quantitative genetic studies on tropical trees have so far focused on fast-growing, light-demanding species, but no information exists on shade-tolerant, slow-growing species. For this study, 27 3-year-old open-pollinated families of the Neotropical shade-tolerant rainforest tree Sextonia rubra were measured in semicontrolled conditions for 20 morphological, growth, and photosynthesis traits; the effect of genetic relatedness, habitat of provenance, and mother tree status on seedling traits was analysed. Nine traits displayed significant genetic effects, while mother tree status and habitat effects were not significant (P > 0.05) for an y trait. Estimated heritability varied between 0.14 and 0.28, with growth-related traits having the highest values. Additive genetic variation correlated positively with nonheritable variation, suggesting that ecological-evolutionary factors increasing or decreasing additive genetic variance may also affect nonheritable variation in the same direction. Our results suggest that quantitative genetic variability should be taken into account in ecological studies on, and in the management of, natural tropical rainforests; further research is needed to investigate genetic x environment interactions, in particular from the point of view of the genetic response of shade-tolerant plant species to variations in light availability. |
| Address |
[Scotti, Ivan; Scotti-Saintagne, Caroline; Bonal, Damien] INRA, Unite Mixte Rech Ecol Forets Guyane, Kourou 97387, French Guiana, Email: ivan.scotti@ecofog.gf |
| Corporate Author |
|
Thesis |
|
| Publisher |
SPRINGER HEIDELBERG |
Place of Publication |
|
Editor |
|
| Language |
|
Summary Language |
|
Original Title |
|
| Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
| Series Volume |
|
Series Issue |
|
Edition |
|
| ISSN |
1614-2942 |
ISBN |
|
Medium |
|
| Area |
|
Expedition |
|
Conference |
|
| Notes |
ISI:000274112600015 |
Approved |
no |
| Call Number |
EcoFoG @ eric.marcon @ |
Serial |
68 |
| Permanent link to this record |
| |
|
| |
| Author |
Odonne, G.; Bourdy, G.; Beauchene, J.; Houel, E.; Stien, D.; Chevolot, L.; Deharo, E. |
| Title |
From Tonic-cups to Bitter-cups: Kwasi bita beker from Suriname Determination, past and present use of an ancient galenic artefact |
Type |
Journal Article |
| Year |
2007 |
Publication |
Journal of Ethnopharmacology |
Abbreviated Journal |
J. Ethnopharmacol. |
| Volume |
110 |
Issue |
2 |
Pages |
318-322 |
| Keywords |
Tonic-cup; Bitter-cup; Quassia amara; Suriname; traditional remedy |
| Abstract |
In the main markets of Paramaribo (Suriname), many stands offer what is locally called “Bitter-cups”, or “Kwasi bita beker”, small footed-cups, roughly carved from a whitish wood. The use of these cups is strictly medicinal and it seems to be restricted to Suriname, as they are not found in neighbouring countries (Guyana, French Guiana). The aim of this study was to identify the botanical origin of Bitter-cups still in use in the Saramaka traditional medicine (as information from field people was controversial), and document the ethnopharmacology of this original galenical artefact. Microscopic and high performance liquid chromatography (HPLC) analyses were carried out on Bitter-cup, and anatomical criteria (marginal parenchyma band, size of intervessel and vessel-ray pits, rays width and rays composition, vessels clustering, frequency and size of parenchyma pits) together with HPLC profiles of the macerates showed that the wood cup was similar to Quassia amara L. (Simaroubaceae) wood. Ethnopharmacological investigation indicates that the use of these cups is simply due to the pharmacological properties attributed to “bitters”, and is strongly linked to tradition and cultural attitudes. This study also emphasizes the long lasting use of these cups, now restricted to Suriname only, with almost no variation over one century. (c) 2006 Elsevier Ireland Ltd. All rights reserved. |
| Address |
Univ Toulouse 3, Ctr IRD, UMR 152, F-97323 Cayenne, France, Email: genevieve.bourdy@ird.fr |
| Corporate Author |
|
Thesis |
|
| Publisher |
ELSEVIER IRELAND LTD |
Place of Publication |
|
Editor |
|
| Language |
|
Summary Language |
|
Original Title |
|
| Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
| Series Volume |
|
Series Issue |
|
Edition |
|
| ISSN |
0378-8741 |
ISBN |
|
Medium |
|
| Area |
|
Expedition |
|
Conference |
|
| Notes |
ISI:000245486900012 |
Approved |
no |
| Call Number |
EcoFoG @ eric.marcon @ |
Serial |
166 |
| Permanent link to this record |
| |
|
| |
| Author |
Dejean, A.; Djieto-Lordon, C.; Cereghino, R.; Leponce, M. |
| Title |
Ontogenetic succession and the ant mosaic: An empirical approach using pioneer trees |
Type |
Journal Article |
| Year |
2008 |
Publication |
Basic and Applied Ecology |
Abbreviated Journal |
Basic Appl. Ecol. |
| Volume |
9 |
Issue |
3 |
Pages |
316-323 |
| Keywords |
ant-plant relationships; dynamics of associations; myrmecophytes; species turnover; tropical rainforests |
| Abstract |
Arboreal ant mosaics have been intensively investigated, but what generates these mosaics remains poorly understood. In this paper, we hypothesize that the dynamics of arboreal ant mosaics could be better understood by examining the ontogenetic succession of ants in tropical trees. We used three African pioneer tree species as biological models. Lophira alata (Ochnaceae) is a long-lived species that does not furnish any reward (i.e., extra-floral nectaries [EFNs], shelter) to ants, Anthocleista vogelii (Gentianaceae) bears extremely well-developed EFNs, and Barteria fistulosa (Passifloraceae) is a long-lived myrmecophyte providing both EFNs and domatia. For both L. alata and A. vogelii, we noted a succession of different associated ants as the plants grew and aged. Ground-nesting, arborealforaging ant species were the first associates, followed by arboreal species that build nests with the leaves of their host trees, together with some species nesting opportunistically in pre-existing cavities. Carton-building Crematogaster species were the last in this succession. The presence of EFNs on A. vogelii slows species turnover, demonstrating that the plant exerts some control over its ant associates. The comparison with B. fistulosa, which generally remains associated with the same plant-ant species during its entire ontogeny, highlights the importance of the selective attractiveness of the trees for their associated ants – or, perhaps, the existence of plant filters that screen arriving ants. (C) 2007 Gesellschaft fur Okologie. Published by Elsevier GmbH. All rights reserved. |
| Address |
[Dejean, Alain] CNRS Guyane, UPS 2561, F-97300 Cayenne, France, Email: alain.dejean@wanadoo.fr |
| Corporate Author |
|
Thesis |
|
| Publisher |
ELSEVIER GMBH, URBAN & FISCHER VERLAG |
Place of Publication |
|
Editor |
|
| Language |
|
Summary Language |
|
Original Title |
|
| Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
| Series Volume |
|
Series Issue |
|
Edition |
|
| ISSN |
1439-1791 |
ISBN |
|
Medium |
|
| Area |
|
Expedition |
|
Conference |
|
| Notes |
ISI:000256734600013 |
Approved |
no |
| Call Number |
EcoFoG @ eric.marcon @ |
Serial |
138 |
| Permanent link to this record |
| |
|
| |
| Author |
Mortier, F.; Rossi, V.; Guillot, G.; Gourlet-Fleury, S.; Picard, N. |
| Title |
Population dynamics of species-rich ecosystems: The mixture of matrix population models approach |
Type |
Journal Article |
| Year |
2013 |
Publication |
Methods in Ecology and Evolution |
Abbreviated Journal |
Methods Ecol. Evol. |
| Volume |
4 |
Issue |
4 |
Pages |
316-326 |
| Keywords |
Bayesian; Clustering; Mixture models; Population dynamics; Reversible jump Markov chain Monte Carlo; Species-rich ecosystems; Tropical rain forests |
| Abstract |
Matrix population models are widely used to predict population dynamics, but when applied to species-rich ecosystems with many rare species, the small population sample sizes hinder a good fit of species-specific models. This issue can be overcome by assigning species to groups to increase the size of the calibration data sets. However, the species classification is often disconnected from the matrix modelling and from the estimation of matrix parameters, thus bringing species groups that may not be optimal with respect to the predicted community dynamics. We proposed here a method that jointly classified species into groups and fit the matrix models in an integrated way. The model was a special case of mixture with unknown number of components and was cast in a Bayesian framework. An MCMC algorithm was developed to infer the unknown parameters: the number of groups, the group of each species and the dynamics parameters. We applied the method to simulated data and showed that the algorithm efficiently recovered the model parameters. We applied the method to a data set from a tropical rain forest in French Guiana. The mixture matrix model classified tree species into well-differentiated groups with clear ecological interpretations. It also accurately predicted the forest dynamics over the 16-year observation period. Our model and algorithm can straightforwardly be adapted to any type of matrix model, using the life cycle diagram. It can be used as an unsupervised classification technique to group species with similar population dynamics. © 2012 The Authors. Methods in Ecology and Evolution © 2012 British Ecological Society. |
| Address |
Statistics Section IMM, Technical University of Denmark, Copenhagen, Denmark |
| Corporate Author |
|
Thesis |
|
| Publisher |
|
Place of Publication |
|
Editor |
|
| Language |
|
Summary Language |
|
Original Title |
|
| Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
| Series Volume |
|
Series Issue |
|
Edition |
|
| ISSN |
2041210x (Issn) |
ISBN |
|
Medium |
|
| Area |
|
Expedition |
|
Conference |
|
| Notes |
Export Date: 16 April 2013; Source: Scopus; :doi 10.1111/2041-210x.12019; Language of Original Document: English; Correspondence Address: Mortier, F.; CIRAD, UPR Bsef, Montpellier, 34398, France; email: frederic.mortier@cirad.fr |
Approved |
no |
| Call Number |
EcoFoG @ webmaster @ |
Serial |
480 |
| Permanent link to this record |
| |
|
| |
| Author |
Leba, L.-J.; Popovici, J.; Estevez, Y.; Pelleau, S.; Legrand, E.; Musset, L.; Duplais, C. |
| Title |
Antiplasmodial activities of dyes against Plasmodium falciparum asexual and sexual stages: Contrasted uptakes of triarylmethanes Brilliant green, Green S (E142), and Patent Blue V (E131) by erythrocytes |
Type |
Journal Article |
| Year |
2017 |
Publication |
International Journal for Parasitology: Drugs and Drug Resistance |
Abbreviated Journal |
International Journal for Parasitology: Drugs and Drug Resistance |
| Volume |
7 |
Issue |
3 |
Pages |
314-320 |
| Keywords |
Antimalarial dyes; Brilliant green; Drug uptake; Food dyes; Transmission blocking; Triarylmethanes |
| Abstract |
The search for safe antimalarial compounds acting against asexual symptom-responsible stages and sexual transmission-responsible forms of Plasmodium species is one of the major challenges in malaria elimination programs. So far, among current drugs approved for human use, only primaquine has transmission-blocking activity. The discovery of small molecules targeting different Plasmodium falciparum life stages remains a priority in antimalarial drug research. In this context, several independent studies have recently reported antiplasmodial and transmission-blocking activities of commonly used stains, dyes and fluorescent probes against P. falciparum including chloroquine-resistant isolates. Herein we have studied the antimalarial activities of dyes with different scaffold and we report that the triarylmethane dye (TRAM) Brilliant green inhibits the growth of asexual stages (IC50 ≤ 2 μM) and has exflagellation-blocking activity (IC50 ≤ 800 nM) against P. falciparum reference strains (3D7, 7G8) and chloroquine-resistant clinical isolate (Q206). In a second step we have investigated the antiplasmodial activities of two polysulfonated triarylmethane food dyes. Green S (E142) is weakly active against P. falciparum asexual stage (IC50 ≃ 17 μM) whereas Patent Blue V (E131) is inactive in both antimalarial assays. By applying liquid chromatography techniques for the culture supernatant analysis after cell washings and lysis, we report the detection of Brilliant green in erythrocytes, the selective uptake of Green S (E142) by infected erythrocytes, whereas Patent Blue V (E131) could not be detected within non-infected and 3D7-infected erythrocytes. Overall, our results suggest that two polysulfonated food dyes might display different affinity with transporters or channels on infected RBC membrane. © 2017 The Authors |
| Address |
Research Unit of Genetics and Genomics of Insect Vectors Institut Pasteur, Paris, France |
| Corporate Author |
|
Thesis |
|
| Publisher |
|
Place of Publication |
|
Editor |
|
| Language |
|
Summary Language |
|
Original Title |
|
| Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
| Series Volume |
|
Series Issue |
|
Edition |
|
| ISSN |
|
ISBN |
|
Medium |
|
| Area |
|
Expedition |
|
Conference |
|
| Notes |
Export Date: 18 September 2017 |
Approved |
no |
| Call Number |
EcoFoG @ webmaster @ |
Serial |
765 |
| Permanent link to this record |
