Sebbenn, A. M., Blanc-Jolivet, C., Mader, M., Meyer-Sand, B. R. V., Paredes-Villanueva, K., Honorio Coronado, E. N., et al. (2019). Nuclear and plastidial SNP and INDEL markers for genetic tracking studies of Jacaranda copaia. Conserv. Gen. Res., 11(3), 341–343.
Abstract: Nuclear and plastidial single nucleotide polymorphism (SNP) and INDEL markers were developed using restriction associated DNA sequencing (RADSeq) and low coverage MiSeq genome sequencing for population genetics and timber tracking purposes in the Neotropical timber species Jacaranda copaia. We used 407 nuclear SNPs, 29 chloroplast, and 31 mitochondrial loci to genotype 92 individuals from Brazil, Bolivia, French Guiana, and Peru. Based on high amplification rates and genetic differentiation among populations, 113 nuclear SNPs, 11 chloroplast, and 4 mitochondrial loci were selected, and their use validated for genetic tracking of timber origin.
Keywords: DNA fingerprints; Geographical origin; Jacaranda copaia; MassARRAY; MiSeq; RADSeq; Tropical timber
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Teixeia, G. A., de Aguiar, H. J. A. C., Petitclerc, F., Orivel, J., Lopes, D. M., & Barros, L. A. C. (2021). Evolutionary insights into the genomic organization of major ribosomal DNA in ant chromosomes. Insect Molecular Biology, 30(3), 340–354.
Abstract: The major rDNA genes are composed of tandem repeats and are part of the nucleolus organizing regions (NORs). They are highly conserved and therefore useful in understanding the evolutionary patterns of chromosomal locations. The evolutionary dynamics of the karyotype may affect the organization of rDNA genes within chromosomes. In this study, we physically mapped 18S rDNA genes in 13 Neotropical ant species from four subfamilies using fluorescence in situ hybridization. Furthermore, a survey of published rDNA cytogenetic data for 50 additional species was performed, which allowed us to detect the evolutionary patterns of these genes in ant chromosomes. Species from the Neotropical, Palearctic, and Australian regions, comprising a total of 63 species from 19 genera within six subfamilies, were analysed. Most of the species (48 out of 63) had rDNA genes restricted to a single chromosome pair in their intrachromosomal regions. The position of rDNA genes within the chromosomes appears to hinder their dispersal throughout the genome, as translocations and ectopic recombination are uncommon in intrachromosomal regions because they can generate meiotic abnormalities. Therefore, rDNA genes restricted to a single chromosome pair seem to be a plesiomorphic feature in ants, while multiple rDNA sites, observed in distinct subfamilies, may have independent origins in different genera.
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Ruelle, J., Beauchene, J., Yamamoto, H., & Thibaut, B. (2011). Variations in physical and mechanical properties between tension and opposite wood from three tropical rainforest species. Wood Sci. Technol., 45(2), 339–357.
Abstract: Growth strains were measured in situ in nine trees of three species from a French Guiana tropical rainforest in a clearly active verticality restoration process. The aim was to detect tension wood within the samples. Wood specimens were cut in the vicinity of the growth strain measurements in order to determine the microfibril angle and some mechanical and physical properties. As suspected, tensile growth strain was much higher in tension wood zones, as shown by the slightly higher longitudinal modulus of elasticity. Conversely, tension wood showed reduced compression strength. Longitudinal shrinkage was much higher in tension wood than in opposite wood. Clear relationships between the microfibril angle and longitudinal properties were noted in comparison (i) with those observed in gymnosperm compression wood and (ii) with expected relationships from the organization of wood fibres cell wall structure.
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Falkowski, M., Jahn-Oyac, A., Ferrero, E., Issaly, J., Eparvier, V., Girod, R., et al. (2016). Assessment of A Simple Compound-Saving Method to Study Insecticidal Activity of Natural Extracts and Pure Compounds Against Mosquito Larvae. Journal of the American Mosquito Control Association, 32(4), 337–340.
Abstract: Research on natural insecticides has intensified with the spread of resistance to chemicals among insects, particularly disease vectors. To evaluate compounds, the World Health Organization (WHO) has published standardized procedures. However, those may be excessively compound-consuming when it comes to assessing the activity of natural extracts and pure compounds isolated in limited amount. As part of our work on the discovery of new mosquito larvicides from Amazonian plants, we developed a compound-saving assay in 5-ml glass tubes instead of WHO larval 100-ml cups. Comparing activity of synthetic and natural chemicals validated the glass tube assay. Raw data, lethal doses that kill 50% (LD50) and 90% (LD90) at 24 and 48 h, were highly correlated (0.68 < R2 < 0.96, P < 0.001, Pearson test) between cups and tubes. It was also established that 10 tubes (N = 50 larvae) provided the same level of sensitivity as 20 tubes (N = 100). This method proved suitable for rapid screening of natural extracts and molecules, identifying active compounds using 10 times less material than in the WHO protocol. © 2016 by The American Mosquito Control Association, Inc.
Keywords: Mosquitoes; natural insecticides; screening method
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Tysklind, N., Blanc-Jolivet, C., Mader, M., Meyer-Sand, B. R. V., Paredes-Villanueva, K., Honorio Coronado, E. N., et al. (2019). Development of nuclear and plastid SNP and INDEL markers for population genetic studies and timber traceability of Carapa species. Conserv. Gen. Res., 11(3), 337–339.
Abstract: Low coverage MiSeq genome sequencing and restriction associated DNA sequencing (RADseq) were used to identify nuclear and plastid SNP and INDEL genetic markers in Carapa guianensis. 261 genetic markers including 237 nuclear SNPs, 22 plastid SNPs, and 2 plastid INDELs are described based on 96 genotyped individuals from French Guiana, Brazil, Peru, and Bolivia. The best 117 SNPs for identifying population structure and performing individual assignment are assembled into four multiplexes for MassARRAY genotyping.
Keywords: Carapa guianensis; Carapa surinamensis; DNA-fingerprints; Geographical origin; MassARRAY; MiSeq; RADSeq; Tropical timber
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Phillips, P. D., de Azevedo, C. P., Degen, B., Thompson, I. S., Silva, J. N. M., & van Gardingen, P. R. (2004). An individual-based spatially explicit simulation model for strategic forest management planning in the eastern Amazon. Ecol. Model., 173(4), 335–354.
Abstract: A model to simulate the ecological processes of tree growth, mortality and recruitment, and the processes of forest management, in the terra firme forests of the eastern Amazon is described. It is implemented within the SYMFOR (http://www.symfor.org) framework. It is based on measurements of all trees that have a diameter greater than 5 cm from experimental plots in the Jari Cellulose and Tapajos National Forest areas over a 16-year period. Ten species groups are used to describe the natural processes affecting tree behaviour. Growth rates are calculated for each species group using the tree diameter and a competition index. Mortality and recruitment are simulated as stochastic processes. Recruitment probability is based on the predicted growth rate of a hypothetical tree. Options exist to vary the human interaction with the forest reflecting forest management decisions, as for other SYMFOR models. Model evaluation compares the performance of the model with data describing forest recovery for 16 years following logging. The model was applied to simulate current forest management practice in the Brazilian Amazon, with 40 m(3) ha(-1) of timber extracted with a cutting cycle of 30 years. Results show that yields are sustained for three harvests following the first logging of primary forest, but that the composition of timber moves towards lightwooded species rather than hardwooded. The predicted size of extracted trees decreases and the number of trees extracted increases with successive harvests, leading to a prediction of increased costs and lower profits for the logging company despite constant yields. The standing volume of all trees just before harvest is reduced by 15% over 150 years, with pioneer species becoming increasingly prevalent in the stand. The model, in the SYMFOR framework, can be used to help understand the differences between alternative forest management strategies in the Brazilian Amazon. Such knowledge is required to improve forest management, regulation and certification, and help to conserve the worlds largest remaining tropical forest. (C) 2003 Elsevier B.V. All rights reserved.
Keywords: model; individual; tropical forest; tree; spatial; Brazil; Amazon; competition; species grouping; management; certification; regulation
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Barabe, D., Cuerrier, A., & Quilichini, A. (2012). Botanical gardens: Between science and commercialization. Natures Sciences Societes, 20(3), 334–342.
Abstract: Les jardins botaniques: Entre science et commercialisation.
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Marcon, E., & Herault, B. (2015). Decomposing phylodiversity. Methods in Ecology and Evolution, 6(3), 333–339.
Abstract: Measuring functional or phylogenetic diversity is the object of an active literature. The main issues to address are relating measures to a clear conceptual framework, allowing unavoidable estimation-bias correction and decomposing diversity along spatial scales. We provide a general mathematical framework to decompose measures of species-neutral, phylogenetic or functional diversity into α and β components. We first unify the definitions of phylogenetic and functional entropy and diversity as a generalization of HCDT entropy and Hill numbers when an ultrametric tree is considered. We then derive the decomposition of diversity. We propose a bias correction of the estimates allowing meaningful computation from real, often undersampled communities. Entropy can be transformed into true diversity, that is an effective number of species or communities. Estimators of α- and β-entropy, phylogenetic and functional entropy are provided. Proper definition and estimation of diversity is the first step towards better understanding its underlying ecological and evolutionary mechanisms. © 2015 British Ecological Society.
Keywords: Biodiversity; Entropy; Functional diversity; Phylogenetic diversity
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Rodríguez-Pérez, H., Hilaire, S., & Mesléard, F. (2016). Temporary pond ecosystem functioning shifts mediated by the exotic red swamp crayfish (Procambarus clarkii): a mesocosm study. Hydrobiologia, 767(1), 333–345.
Abstract: Temporary ponds, acknowledged for their conservation value, are colonized by the invasive crayfish Procambarus clarkii. We have tested the consequences of this colonization for the ecosystem under two contrasted scenarios: one single individual arrival or three individuals arrival. We recreated the temporary pond ecosystem in 1 m2 tanks to investigate the impact of the two crayfish densities. We studied the macrophyte community composition and abundance, chlorophyll a and total suspended solids concentrations, and the diversity and functional composition of micro-crustacean and macro-invertebrate communities. We observed a reduction of macrophyte biomass in experimental crayfish mesocosms in comparison with control tanks, nearly 80 and 40% less in 3 and 1 crayfish/m2 tanks, respectively. The macrophyte community shifted, followed by a filamentous algae development, an increase of bare sediment and turbidity in crayfish tanks. The macro-invertebrate community suffered a richness loss of 28 and 22%, in 3 and 1 crayfish/m2 tanks, respectively. Functionally, macro-invertebrate diversity reduction most strongly affected the grazer, detritivore and predator trophic groups. Microcrustaceans seemed not to be affected by the introduction of the crayfish. The introduction of the crayfish greatly altered the ecosystem structure and subsequently the ecosystem functioning. © 2015, Springer International Publishing Switzerland.
Keywords: Ecosystem functioning; Exotic crayfish; Procambarus clarkii; Temporary pond
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Honorio Coronado, E. N., Blanc-Jolivet, C., Mader, M., García-Dávila, C. R., Sebbenn, A. M., Meyer-Sand, B. R. V., et al. (2019). Development of nuclear and plastid SNP markers for genetic studies of Dipteryx tree species in Amazonia. Conserv. Genet. Res., 11(3), 333–336.
Abstract: We developed nuclear and plastid single nucleotide polymorphism (SNP) and insertion/deletion (INDEL) markers for Dipteryx species using a combination of restriction associated DNA sequencing (RADSeq) and low coverage MiSeq genome sequencing. Of the total 315 loci genotyped using a MassARRAY platform, 292 loci were variable and polymorphic among the 73 sampled individuals from French Guiana, Brasil, Peru, and Bolivia. A final set of 56 nuclear SNPs, 26 chloroplast SNPs, 2 chloroplast INDELs, and 32 mitochondrial SNPs identifying significant population structure was developed. This set of loci will be useful for studies on population genetics of Dipteryx species in Amazonia.
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