@Article{Garcia-Davila_etal2020,
author="Garcia-Davila, C.
and Aldana Gomero, D.
and Renno, J.-F.
and Diaz Soria, R.
and Hidalgo Pizango, G.
and Flores Llampazo, G.
and Castro-Ruiz, D.
and Mejia de Loayza, E.
and Angulo Chavez, C.
and Mader, M.
and Tysklind, N.
and Paredes-Villanueva, K.
and del Castillo Torres, D.
and Degen, B.
and Honorio Coronado, E.N.",
title="Molecular evidence for three genetic species of Dipteryx in the Peruvian Amazon",
journal="Genetica",
year="2020",
publisher="Springer",
volume="148",
number="1",
pages="1--11",
optkeywords="D. micrantha",
optkeywords="Dipteryx charapilla",
optkeywords="Genetic diversity",
optkeywords="Microsatellites",
optkeywords="Sequencing",
optkeywords="Shihuahuaco",
optkeywords="microsatellite DNA",
optkeywords="plant DNA",
optkeywords="allele",
optkeywords="Dipteryx",
optkeywords="DNA sequence",
optkeywords="genetic variation",
optkeywords="genetics",
optkeywords="genotype",
optkeywords="haplotype",
optkeywords="Peru",
optkeywords="phylogeny",
optkeywords="plastid",
optkeywords="river",
optkeywords="species difference",
optkeywords="Alleles",
optkeywords="DNA",
optkeywords="Plant",
optkeywords="Haplotypes",
optkeywords="Microsatellite Repeats",
optkeywords="Plastids",
optkeywords="Rivers",
optkeywords="Sequence Analysis",
optkeywords="Species Specificity",
abstract="There is a high international demand for timber from the genus Dipteryx, or {\textquoteleft}{\textquoteleft}shihuahuaco{\textquoteright}{\textquoteright} as it is known in Peru. Developing tools that allow the identification and discrimination of Dipteryx species is therefore important for supporting management of natural populations and to underpin legal trade of its timber. The objective of this study was the molecular characterization of Dipteryx species in the Peruvian Amazonia. Two plastid regions (cpDNA: trnH--psbA and matK) were sequenced and 11 microsatellite markers (nDNA) were genotyped for 32 individuals identified as Dipteryx charapilla, D. micrantha morphotype 1 and D. micrantha morphotype 2. Using the concatenated sequences of the plastid genes, we identified ten haplotypes that were not shared between the species or between the D. micrantha morphotypes. Haplotypic diversity was greater in D. micrantha morphotype 2 and D. charapilla than in D. micrantha morphotype 1, which presented only one haplotype with a wide distribution in Peru. The microsatellites allowed the discrimination of the same three clades and identified diagnostic alleles for each clade. These results allowed us to demonstrate that the two morphotypes of D. micrantha are different at both the plastid and nuclear markers, which supports the existence of three genetically distinct species in Peru. This study provides information for the genetic discrimination of Dipteryx species and emphasises the importance of conserving the genetic variability of this genus in the Peruvian Amazonia. {\textcopyright} 2019, Springer Nature Switzerland AG.",
optnote="exported from refbase (http://php.ecofog.gf/refbase/show.php?record=990), last updated on Mon, 08 Feb 2021 11:12:36 -0300",
issn="00166707 (Issn)",
doi="10.1007/s10709-019-00082-2"
}