TY  - JOUR
AU  - Touchard, A.
AU  - Aili, S.R.
AU  - Téné, N.
AU  - Barassé, V.
AU  - Klopp, C.
AU  - Dejean, A.
AU  - Kini, R.M.
AU  - Mrinalini
AU  - Coquet, L.
AU  - Jouenne, T.
AU  - Lefranc, B.
AU  - Leprince, J.
AU  - Escoubas, P.
AU  - Nicholson, G.M.
AU  - Treilhou, M.
AU  - Bonnafé, E.
PY  - 2020//
TI  - Venom Peptide Repertoire of the European Myrmicine Ant Manica rubida: Identification of Insecticidal Toxins
T2  - J. Proteome Res.
JO  - Journal of proteome research
SP  - 1800
EP  - 1811
VL  - 19
IS  - 4
PB  - NLM (Medline)
KW  - glycosylated toxin
KW  - peptidome
KW  - polycationic α-helix
KW  - predation
KW  - pyroglutamate
KW  - reversible neurotoxicity
N2  - Using an integrated transcriptomic and proteomic approach, we characterized the venom peptidome of the European red ant, Manica rubida. We identified 13 "myrmicitoxins" that share sequence similarities with previously identified ant venom peptides, one of them being identified as an EGF-like toxin likely resulting from a threonine residue modified by O-fucosylation. Furthermore, we conducted insecticidal assays of reversed-phase HPLC venom fractions on the blowfly Lucilia caesar, permitting us to identify six myrmicitoxins (i.e., U3-, U10-, U13-, U20-MYRTX-Mri1a, U10-MYRTX-Mri1b, and U10-MYRTX-Mri1c) with an insecticidal activity. Chemically synthesized U10-MYRTX-Mri1a, -Mri1b, -Mri1c, and U20-MYRTX-Mri1a irreversibly paralyzed blowflies at the highest doses tested (30-125 nmol·g-1). U13-MYRTX-Mri1a, the most potent neurotoxic peptide at 1 h, had reversible effects after 24 h (150 nmol·g-1). Finally, U3-MYRTX-Mri1a has no insecticidal activity, even at up to 55 nmol·g-1. Thus, M. rubida employs a paralytic venom rich in linear insecticidal peptides, which likely act by disrupting cell membranes.
SN  - 15353907 (Issn)
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85082979218&doi=10.1021%2facs.jproteome.0c00048&partnerID=40&md5=03e933a09635e5b8227ffebddc7f6536
UR  - http://dx.doi.org/10.1021/acs.jproteome.0c00048
N1  - Export Date: 20 April 2020
ID  - Touchard_etal2020
ER  -