%0 Journal Article
%T Biosynthetic investigation of γ-lactones in Sextonia rubra wood using in situ TOF-SIMS MS/MS imaging to localize and characterize biosynthetic intermediates
%A Fu, T.
%A Houel, E.
%A Amusant, N.
%A Touboul, D.
%A Genta-Jouve, G.
%A Della-Negra, S.
%A Fisher, G.L.
%A Brunelle, A.
%A Duplais, C.
%J Scientific Reports
%D 2019
%V 9
%I Nature Publishing Group
%@ 20452322 (Issn)
%F Fu_etal2019
%O exported from refbase (http://php.ecofog.gf/refbase/show.php?record=866), last updated on Tue, 20 Aug 2019 14:20:02 -0300
%X Molecular analysis by parallel tandem mass spectrometry (MS/MS) imaging contributes to the in situ characterization of biosynthetic intermediates which is crucial for deciphering the metabolic pathways in living organisms. We report the first use of TOF-SIMS MS/MS imaging for the cellular localization and characterization of biosynthetic intermediates of bioactive γ-lactones rubrynolide and rubrenolide in the Amazonian tree Sextonia rubra (Lauraceae). Five γ-lactones, including previously reported rubrynolide and rubrenolide, were isolated using a conventional approach and their structural characterization and localization at a lateral resolution of ~400 nm was later achieved using TOF-SIMS MS/MS imaging analysis. 2D/3D MS imaging at subcellular level reveals that putative biosynthetic γ-lactones intermediates are localized in the same cell types (ray parenchyma cells and oil cells) as rubrynolide and rubrenolide. Consequently, a revised metabolic pathway of rubrynolide was proposed, which involves the reaction between 2-hydroxysuccinic acid and 3-oxotetradecanoic acid, contrary to previous studies suggesting a single polyketide precursor. Our results provide insights into plant metabolite production in wood tissues and, overall, demonstrate that combining high spatial resolution TOF-SIMS imaging and MS/MS structural characterization offers new opportunities for studying molecular and cellular biochemistry in plants. © 2019, The Author(s).
%U https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061497447&doi=10.1038%2fs41598-018-37577-5&partnerID=40&md5=3462e9e21023f28f1cfb138bc4b7bccc
%U http://php.ecofog.gf/refbase/files/fu/2019/866_Fu_etal2019.pdf
%U http://dx.doi.org/10.1038/s41598-018-37577-5
%P 1928