@Article{Fu_etal2019,
author="Fu, T.
and Houel, E.
and Amusant, N.
and Touboul, D.
and Genta-Jouve, G.
and Della-Negra, S.
and Fisher, G.L.
and Brunelle, A.
and Duplais, C.",
title="Biosynthetic investigation of $\gamma$-lactones in Sextonia rubra wood using in situ TOF-SIMS MS/MS imaging to localize and characterize biosynthetic intermediates",
journal="Scientific Reports",
year="2019",
publisher="Nature Publishing Group",
volume="9",
pages="1928",
abstract="Molecular analysis by parallel tandem mass spectrometry (MS/MS) imaging contributes to the in situ characterization of biosynthetic intermediates which is crucial for deciphering the metabolic pathways in living organisms. We report the first use of TOF-SIMS MS/MS imaging for the cellular localization and characterization of biosynthetic intermediates of bioactive $\gamma$-lactones rubrynolide and rubrenolide in the Amazonian tree Sextonia rubra (Lauraceae). Five $\gamma$-lactones, including previously reported rubrynolide and rubrenolide, were isolated using a conventional approach and their structural characterization and localization at a lateral resolution of {\textasciitilde}400 nm was later achieved using TOF-SIMS MS/MS imaging analysis. 2D/3D MS imaging at subcellular level reveals that putative biosynthetic $\gamma$-lactones intermediates are localized in the same cell types (ray parenchyma cells and oil cells) as rubrynolide and rubrenolide. Consequently, a revised metabolic pathway of rubrynolide was proposed, which involves the reaction between 2-hydroxysuccinic acid and 3-oxotetradecanoic acid, contrary to previous studies suggesting a single polyketide precursor. Our results provide insights into plant metabolite production in wood tissues and, overall, demonstrate that combining high spatial resolution TOF-SIMS imaging and MS/MS structural characterization offers new opportunities for studying molecular and cellular biochemistry in plants. {\textcopyright} 2019, The Author(s).",
optnote="exported from refbase (http://php.ecofog.gf/refbase/show.php?record=866), last updated on Tue, 20 Aug 2019 14:20:02 -0300",
issn="20452322 (Issn)",
doi="10.1038/s41598-018-37577-5",
opturl="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061497447&doi=10.1038\%2fs41598-018-37577-5&partnerID=40&md5=3462e9e21023f28f1cfb138bc4b7bccc",
file=":http://php.ecofog.gf/refbase/files/fu/2019/866\textit{Fu}etal2019.pdf:PDF"
}