TY  - JOUR
AU  - Aili, S.R.
AU  - Touchard, A.
AU  - Petitclerc, F.
AU  - Dejean, A.
AU  - Orivel, J.
AU  - Padula, M.P.
AU  - Escoubas, P.
AU  - Nicholson, G.M.
PY  - 2017//
TI  - Combined Peptidomic and Proteomic Analysis of Electrically Stimulated and Manually Dissected Venom from the South American Bullet Ant Paraponera clavata
T2  - J. Proteome Res.
JO  - Journal of Proteome Research
SP  - 1339
EP  - 1351
VL  - 16
IS  - 3
PB  - American Chemical Society
N2  - Ants have evolved venoms rich in peptides and proteins used for predation, defense, and communication. However, they remain extremely understudied due to the minimal amount of venom secreted by each ant. The present study investigated the differences in the proteome and peptidome of the venom from the bullet ant, Paraponera clavata. Venom samples were collected from a single colony either by manual venom gland dissection or by electrical stimulation and were compared using proteomic methods. Venom proteins were separated by 2D-PAGE and identified by nanoLC-ESI-QTOF MS/MS. Venom peptides were initially separated using C18 reversed-phase high-performance liquid chromatography, then analyzed by MALDI-TOF MS. The proteomic analysis revealed numerous proteins that could be assigned a biological function (total 94), mainly as toxins, or roles in cell regulation and transport. This investigation found that ca. 73% of the proteins were common to venoms collected by the two methods. The peptidomic analysis revealed a large number of peptides (total 309) but with <20% shared by the two collection methods. There was also a marked difference between venoms obtained by venom gland dissection from different ant colonies. These findings demonstrate the rich composition and variability of P. clavata venom.
SN  - 1535-3893
UR  - http://dx.doi.org/10.1021/acs.jproteome.6b00948
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ID  - Aili_etal2017
ER  -