TY  - JOUR
AU  - Zinger, L.
AU  - Chave, J.
AU  - Coissac, E.
AU  - Iribar, A.
AU  - Louisanna, E.
AU  - Manzi, S.
AU  - Schilling, V.
AU  - Schimann, H.
AU  - Sommeria-Klein, G.
AU  - Taberlet, P.
PY  - 2016//
TI  - Extracellular DNA extraction is a fast, cheap and reliable alternative for multi-taxa surveys based on soil DNA
T2  - Soil Biology and Biochemistry
JO  - Soil Biology and Biochemistry
SP  - 16
EP  - 19
VL  - 96
KW  - DNA extraction protocol
KW  - DNA metabarcoding
KW  - Multi-taxa biodiversity
KW  - Tropical forest
N2  - DNA metabarcoding on soil samples is increasingly used for large-scale and multi-taxa biodiversity studies. However, DNA extraction may be a major bottleneck for such wide uses. It should be cost/time effective and allow dealing with large sample volumes so as to maximise the representativeness of both micro- and macro-organisms diversity. Here, we compared the performances of a fast and cheap extracellular DNA extraction protocol with a total DNA extraction method in retrieving bacterial, eukaryotic and plant diversity from tropical soil samples of ca. 10 g. The total DNA extraction protocol yielded more high-quality DNA. Yet, the extracellular DNA protocol provided similar diversity assessments although it presented some differences in clades relative abundance and undersampling biases. We argue that extracellular DNA is a good compromise between cost, labor, and accuracy for high-throughput DNA metabarcoding studies of soil biodiversity. © 2016 Elsevier Ltd.
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-84957080342&partnerID=40&md5=0ee152c08c6f2a42837a8d9097489c12
N1  - Export Date: 17 February 2016
ID  - Zinger_etal2016
ER  -