PT Journal
AU Zinger, L
   Chave, J
   Coissac, E
   Iribar, A
   Louisanna, E
   Manzi, S
   Schilling, V
   Schimann, H
   Sommeria-Klein, G
   Taberlet, P
TI Extracellular DNA extraction is a fast, cheap and reliable alternative for multi-taxa surveys based on soil DNA
SO Soil Biology and Biochemistry
JI Soil Biology and Biochemistry
PY 2016
BP 16
EP 19
VL 96
DE DNA extraction protocol; DNA metabarcoding; Multi-taxa biodiversity; Tropical forest
AB DNA metabarcoding on soil samples is increasingly used for large-scale and multi-taxa biodiversity studies. However, DNA extraction may be a major bottleneck for such wide uses. It should be cost/time effective and allow dealing with large sample volumes so as to maximise the representativeness of both micro- and macro-organisms diversity. Here, we compared the performances of a fast and cheap extracellular DNA extraction protocol with a total DNA extraction method in retrieving bacterial, eukaryotic and plant diversity from tropical soil samples of ca. 10 g. The total DNA extraction protocol yielded more high-quality DNA. Yet, the extracellular DNA protocol provided similar diversity assessments although it presented some differences in clades relative abundance and undersampling biases. We argue that extracellular DNA is a good compromise between cost, labor, and accuracy for high-throughput DNA metabarcoding studies of soil biodiversity. © 2016 Elsevier Ltd.
ER