TY  - JOUR
AU  - Touchard, A.
AU  - Dauvois, M.
AU  - Arguel, M.-J.
AU  - Petitclerc, F.
AU  - Leblanc, M.
AU  - Dejean, A.
AU  - Orivel, J.
AU  - Nicholson, G.M.
AU  - Escoubas, P.
PY  - 2014//
TI  - Elucidation of the unexplored biodiversity of ant venom peptidomes via MALDI-TOF mass spectrometry and its application for chemotaxonomy
T2  - J. Proteomics
JO  - Journal of Proteomics
SP  - 217
EP  - 231
VL  - 105
PB  - Elsevier
KW  - Ant venom
KW  - Chemotaxonomy
KW  - Maldi-Tof Ms
KW  - Peptide
KW  - Peptidome
KW  - Ponerinae
KW  - cytochrome c oxidase
KW  - ant
KW  - article
KW  - biodiversity
KW  - correlational study
KW  - DNA sequence
KW  - French Guiana
KW  - Hymenoptera
KW  - matrix assisted laser desorption ionization time of flight mass spectrometry
KW  - mitochondrial gene
KW  - nonhuman
KW  - Odontomachus biumbonatus
KW  - Odontomachus haematodus
KW  - Odontomachus hastatus
KW  - Odontomachus mayi
KW  - Odontomachus scalptus
KW  - Pachcondyla apicalis
KW  - Pachcondyla arhuaca
KW  - Pachcondyla commutata
KW  - Pachcondyla constricta
KW  - Pachcondyla crassinola
KW  - Pachcondyla goeldii
KW  - Pachcondyla inversa
KW  - Pachcondyla marginata
KW  - Pachcondyla procidua
KW  - Pachcondyla stigma
KW  - Pachcondyla verenae
KW  - Pachcondyla villosa
KW  - peptidomics
KW  - phylogeny
KW  - priority journal
KW  - Animalia
KW  - Formicidae
KW  - Odontomachus
KW  - Pachycondyla
KW  - Pachycondyla apicalis
KW  - Pachycondyla stigma
N2  - The rise of integrative taxonomy, a multi-criteria approach used in characterizing species, fosters the development of new tools facilitating species delimitation. Mass spectrometric (MS) analysis of venom peptides from venomous animals has previously been demonstrated to be a valid method for identifying species. Here we aimed to develop a rapid chemotaxonomic tool for identifying ants based on venom peptide mass fingerprinting. The study focused on the biodiversity of ponerine ants (Hymenoptera: Formicidae: Ponerinae) in French Guiana. Initial experiments optimized the use of automated matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to determine variations in the mass profiles of ant venoms using several MALDI matrices and additives. Data were then analyzed via a hierarchical cluster analysis to classify the venoms of 17 ant species. In addition, phylogenetic relationships were assessed and were highly correlated with methods using DNA sequencing of the mitochondrial gene cytochrome c oxidase subunit 1. By combining a molecular genetics approach with this chemotaxonomic approach, we were able to improve the accuracy of the taxonomic findings to reveal cryptic ant species within species complexes. This chemotaxonomic tool can therefore contribute to more rapid species identification and more accurate taxonomies. Biological significance: This is the first extensive study concerning the peptide analysis of the venom of both Pachycondyla and Odontomachus ants. We studied the venoms of 17 ant species from French Guiana that permitted us to fine-tune the venom analysis of ponerine ants via MALDI-TOF mass spectrometry. We explored the peptidomes of crude ant venom and demonstrated that venom peptides can be used in the identification of ant species. In addition, the application of this novel chemotaxonomic method combined with a parallel genetic approach using COI sequencing permitted us to reveal the presence of cryptic ants within both the Pachycondyla apicalis and Pachycondyla stigma species complexes. This adds a new dimension to the search for means of exploiting the enormous biodiversity of venomous ants as a source for novel therapeutic drugs or biopesticides. This article is part of a Special Issue entitled: Proteomics of non-model organisms. © 2014 Elsevier B.V.
SN  - 18767737 (Issn)
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-84901824891&partnerID=40&md5=af32f0452cce4a29375888495cf1c27c
N1  - Cited By (since 1996):1; Export Date: 30 July 2014; Correspondence Address: Touchard, A.; UMR-EcoFoG, Campus Agronomique, BP 316, 97379 Kourou Cedex, France; email: axel.touchard@ecofog.gf; Chemicals/CAS: cytochrome c oxidase, 72841-18-0, 9001-16-5
ID  - Touchard_etal2014
ER  -