@Article{Woolfit_etal2013,
author="Woolfit, M.
and Iturbe-Ormaetxe, I.
and Brownlie, J.C.
and Walker, T.
and Riegler, M.
and Seleznev, A.
and Popovici, J.
and Ranc{\`e}s, E.
and Wee, B.A.
and Pavlides, J.
and Sullivan, M.J.
and Beatson, S.A.
and Lane, A.
and Sidhu, M.
and McMeniman, C.J.
and McGraw, E.A.
and O{\textquoteright}Neill, S.L.",
title="Genomic evolution of the pathogenic Wolbachia strain, wMelPop",
journal="Genome Biology and Evolution",
year="2013",
volume="5",
number="11",
pages="2189--2204",
optkeywords="Endosymbiont",
optkeywords="Evolution",
optkeywords="Genomics",
optkeywords="Wolbachia",
abstract="Most strains of the widespread endosymbiotic bacterium Wolbachia pipientis are benign or behave as reproductive parasites. The pathogenic strain wMelPop is a striking exception, however: it overreplicates in its insect hosts and causes severe life shortening. The mechanism of this pathogenesis is currently unknown. We have sequenced the genomes of three variants of wMelPop and of the closely related nonpathogenic strain wMelCS. We show that the genomes of wMelCS and wMelPop appear to be identical in the nonrepeat regions of the genome and differ detectably only by the triplication of a 19-kb region that is unlikely to be associated with life shortening, demonstrating that dramatic differences in the host phenotype caused by this endosymbiont may be the result of only minor genetic changes. We also compare the genomes of the original wMelPop strain from Drosophila melanogaster and two sequentialderivatives, wMelPop-CLA and wMelPop-PGYP. To develop wMelPop as a novel biocontrol agent, it was first transinfected into and passaged in mosquito cell lines for approximately 3.5 years, generating wMelPop-CLA. This cell line-passaged strain was then transinfected into Aedesaegypti mosquitoes, creating wMelPop-PGYP,which wassequenced after 4yearsin the insecthost. We observe a rapid burst of genomic changes during cell line passaging, but no further mutations were detected after transinfection into mosquitoes, indicating either that host preadaptation had occurred in cell lines, that cell lines are a more selectively permissive environment than animal hosts, or both. Our results provide valuable data on the rates of genomic and phenotypic change in Wolbachia associated with host shifts over short time scales. {\textcopyright} The Author(s) 2013. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.",
optnote="Export Date: 9 February 2014; Source: Scopus; Language of Original Document: English; Correspondence Address: O{\textquoteright}Neill, S.L.; School of Biological Sciences, Monash University, Clayton, VIC, Australia; email: scott.oneill@monash.edu; Funding Details: NIH, National Institutes of Health",
optnote="exported from refbase (http://php.ecofog.gf/refbase/show.php?record=527), last updated on Sun, 09 Feb 2014 02:48:45 -0300",
issn="17596653 (Issn)",
opturl="http://www.scopus.com/inward/record.url?eid=2-s2.0-84892645334&partnerID=40&md5=3ac1dbb8c40a966f96b3c67e2c7d28de",
file=":http://php.ecofog.gf/refbase/files/woolfit/2013/527\textit{Woolfit}etal2013.pdf:PDF"
}