@Article{Houel_etal2014,
author="Houel, E.
and Rodrigues, A.M.S.
and Jahn-Oyac, A.
and Bessi{\`e}re, J.-M.
and Eparvier, V.
and Deharo, E.
and Stien, D.",
title="In vitro antidermatophytic activity of Otacanthus azureus (Linden) Ronse essential oil alone and in combination with azoles",
journal="Journal of Applied Microbiology",
year="2014",
volume="116",
number="2",
pages="288--294",
optkeywords="Antifungal activity",
optkeywords="Azoles",
optkeywords="Dermatophytes",
optkeywords="Essential oil",
optkeywords="Otacanthus azureus",
optkeywords="Synergy",
abstract="Aims: We determined the chemical composition and investigated the antifungal activity of Otacanthus azureus (Linden) Ronse essential oil (EO) against a range of dermatophytes alone or in combination with azole antifungals. Methods and Results: Aerial parts of the plant were steam-distilled and the obtained oil was analysed by gas chromatography/mass spectrometry and 1H-NMR. It was shown to be largely composed of sesquiterpenes, with the main component being $\beta$-copaen-4-$\alpha$-ol. Using broth microdilution techniques, this oil was found to have remarkable in vitro antifungal activities. Minimum inhibitory concentrations as low as 4~$\mu$g ml-1 were recorded. The analysis of the combined effect of the O. azureus EO with azoles using chequerboard assays revealed a synergism between the EO and ketoconazole, fluconazole or itraconazole against Trichophyton mentagrophytes. Notably, the O. azureus essential oil showed low cytotoxicity to VERO cells. Conclusions: The O. azureus essential oil alone or in combination with azoles is a promising antifungal agent in the treatment for human dermatomycoses caused by filamentous fungi. Significance and Impact of the Study: There is much interest in the study of essential oils for the discovery of new antimicrobial drugs. This study has highlighted the antidermatophytic activity of the O. azureus EO. {\textcopyright} 2013 The Society for Applied Microbiology.",
optnote="Export Date: 9 February 2014; Source: Scopus; Coden: Jamif; Language of Original Document: English; Correspondence Address: Hou{\"e}l, E.; CNRS -- UMR Ecologie des For{\^e}ts de Guyane (EcoFoG), Institut Pasteur de la Guyane, 23 Avenue Pasteur, BP6010, 97306 Cayenne Cedex, French Guiana; email: emeline.houel@ecofog.gf",
optnote="exported from refbase (http://php.ecofog.gf/refbase/show.php?record=526), last updated on Mon, 10 Feb 2014 22:21:41 -0300",
issn="13645072 (Issn)",
opturl="http://www.scopus.com/inward/record.url?eid=2-s2.0-84892555516&partnerID=40&md5=dfaf8e446c529fd1e3d5767a5a27d788"
}