PT Journal
AU Ruiz-González, M
   Lauth, J
   Leroy, C
   Jauneau, A
   Gryta, H
   Jargeat, P
   Dejean, A
   Orivel, J
TI An efficient protocol for isolating melanised chaetothyrialean anamorphic fungi associated with plant-ants
SO Journal of Basic Microbiology
PY 2013
BP 98
EP 100
VL 53
IS 1
DE Allomerus ants; Black fungi; Chaetothyriales; Fluorescent staining; Isolation method
AB Because of their ecological characteristics, slow growth rates and the presence of contaminants, Chaetothyriales fungi associated with structures built by tropical plant-ants can be difficult to isolate with standard procedures. Here, we describe an easy-to-use protocol for obtaining pure cultures by using cotton as a first substrate. We have further found by means of fluorescent stains that nuclei concentrate either in young hyphae or in the tips of the hyphae. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
ER