TY  - JOUR
AU  - Odonne, G.
AU  - Herbette, G.
AU  - Eparvier, V.
AU  - Bourdy, G.
AU  - Rojas, R.
AU  - Sauvain, M.
AU  - Stien, D.
PY  - 2011//
TI  - Antileishmanial sesquiterpene lactones from Pseudelephantopus spicatus, a traditional remedy from the Chayahuita Amerindians (Peru). Part III
T2  - J. Ethnopharmacol.
JO  - Journal of Ethnopharmacology
SP  - 875
EP  - 879
VL  - 137
IS  - 1
KW  - Asteraceae
KW  - Chayahuita
KW  - Hirsutinolides
KW  - Leishmaniasis
KW  - Peru
KW  - Pseudelephantopus spicatus
KW  - Traditional remedy
KW  - 8
KW  - 13 diacetylpiptocarphol
KW  - acetyl 13 o ethylpiptocarphol
KW  - amphotericin B
KW  - antileishmanial agent
KW  - plant extract
KW  - Pseudelephantopus spicatus extract
KW  - sesquiterpene lactone derivative
KW  - unclassified drug
KW  - ursolic acid
KW  - amastigote
KW  - article
KW  - biological activity
KW  - Ic 50
KW  - in vitro study
KW  - Leishmania
KW  - medicinal plant
KW  - nonhuman
KW  - traditional medicine
KW  - Leishmania amazonensis
N2  - Ethnopharmacological relevance: The study of traditional remedies used by the Chayahuita, an ethnic group from the Peruvian Amazonia, has prompted us to investigate in detail the ethanolic extract of Pseudelephantopus spicatus (Juss. ex Aubl.) C.F. Baker, which has demonstrated strong biological activity towards Leishmania amazonensis. Our goal was to discover the active compound of this plant-based remedy. Materials and methods: A bioguided fractionation of the crude extract was undertaken based on the biological activity recorded against Leishmania amazonensis axenic amastigotes in in vitro bioassays. Results: Three strongly to moderately active compounds were isolated: two hirsutinolides (the 8,13-diacetyl-piptocarphol and the 8-acetyl-13-O-ethyl-piptocarphol) and ursolic acid. IC50 against Leishmania amazonensis axenic amastigotes are respectively 0.2, 0.37 and 0.99 μM (while IC50 of amphotericin B is 0.41 μM). These compounds have never been isolated from this plant species, and germacranolides have never been identified as potential antileishmanial agents. Conclusions: The compounds isolated from Pseudelephantopus spicatus account for the antileishmanial activity of the plant, thus giving support to its use by the Chayahuita in Peru. © 2011 Elsevier Ireland Ltd All rights reserved.
SN  - 03788741 (Issn)
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-80052031355&partnerID=40&md5=3d857edf1641fb5e735e08bdf7a40b5c
N1  - Export Date: 21 October 2011; Source: Scopus; Coden: Joetd; doi: 10.1016/j.jep.2011.07.008; Language of Original Document: English; Correspondence Address: Odonne, G.; Bureau du CNRS en Guyane, CNRS – UPS 2561, 2 Avenue Gustave Charlery, 97300 Cayenne, France; email: guillaume.odonne@gmail.com; Chemicals/CAS: amphotericin B, 1397-89-3, 30652-87-0; ursolic acid, 77-52-1
ID  - Odonne_etal2011
ER  -