TY  - JOUR
AU  - Fu, T.
AU  - Touboul, D.
AU  - Della-Negra, S.
AU  - Houel, E.
AU  - Amusant, N.
AU  - Duplais, C.
AU  - Fisher, G.L.
AU  - Brunelle, A.
PY  - 2018//
TI  - Tandem Mass Spectrometry Imaging and in Situ Characterization of Bioactive Wood Metabolites in Amazonian Tree Species Sextonia rubra
T2  - Anal. Chem.
JO  - Analytical Chemistry
SP  - 7535
EP  - 7543
VL  - 90
IS  - 12
PB  - American Chemical Society
N2  - Driven by a necessity for confident molecular identification at high spatial resolution, a new time-of-flight secondary ion mass spectrometry (TOF-SIMS) tandem mass spectrometry (tandem MS) imaging instrument has been recently developed. In this paper, the superior MS/MS spectrometry and imaging capability of this new tool is shown for natural product study. For the first time, via in situ analysis of the bioactive metabolites rubrynolide and rubrenolide in Amazonian tree species Sextonia rubra (Lauraceae), we were able both to analyze and to image by tandem MS the molecular products of natural biosynthesis. Despite the low abundance of the metabolites in the wood sample(s), efficient MS/MS analysis of these γ-lactone compounds was achieved, providing high confidence in the identification and localization. In addition, tandem MS imaging minimized the mass interferences and revealed specific localization of these metabolites primarily in the ray parenchyma cells but also in certain oil cells and, further, revealed the presence of previously unidentified γ-lactone, paving the way for future studies in biosynthesis.
SN  - 0003-2700
UR  - https://doi.org/10.1021/acs.analchem.8b01157
UR  - http://dx.doi.org/10.1021/acs.analchem.8b01157
N1  - doi: 10.1021/acs.analchem.8b01157
ID  - Fu_etal2018
ER  -