TY - JOUR AU - Ruiz-González, M.X. AU - Lauth, J. AU - Leroy, C. AU - Jauneau, A. AU - Gryta, H. AU - Jargeat, P. AU - Dejean, A. AU - Orivel, J. PY - 2013// TI - An efficient protocol for isolating melanised chaetothyrialean anamorphic fungi associated with plant-ants JO - Journal of Basic Microbiology SP - 98 EP - 100 VL - 53 IS - 1 KW - Allomerus ants KW - Black fungi KW - Chaetothyriales KW - Fluorescent staining KW - Isolation method N2 - Because of their ecological characteristics, slow growth rates and the presence of contaminants, Chaetothyriales fungi associated with structures built by tropical plant-ants can be difficult to isolate with standard procedures. Here, we describe an easy-to-use protocol for obtaining pure cultures by using cotton as a first substrate. We have further found by means of fluorescent stains that nuclei concentrate either in young hyphae or in the tips of the hyphae. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84872827634&partnerID=40&md5=118f1fbcb56582c6979c30d59afa000b N1 - Export Date: 6 February 2013; Source: Scopus ID - Ruiz-Gonzalez_etal2013 ER -