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Author (up) Abedini, R.; Clair, B.; Pourtahmasi, K.; Laurans, F.; Arnould, O. url  openurl
  Title Cell wall thickening in developing tension wood of artificially bent poplar trees Type Journal Article
  Year 2015 Publication IAWA Journal Abbreviated Journal IAWA Journal  
  Volume 36 Issue 1 Pages 44-57  
  Keywords developing xylem; Gelatinous layer; maturation stress; secondary wall layer; tree biomechanics  
  Abstract Trees can control their shape and resist gravity thanks to their ability to produce wood under tensile stress. This stress is known to be produced during the maturation of wood fibres but the mechanism of its generation remains unclear. This study focuses on the formation of the secondary wall in tension wood produced in artificially tilted poplar saplings. Thickness of secondary wall layer (SL) and gelatinous layer (GL) were measured from cambium to mature wood in several trees sampled at different times after tilting. Measurements on wood fibres produced before tilting show the progressive increase of secondary wall thickness during the growing season. After the tilting date, SL thickness decreased markedly from normal wood to tension wood while the total thickness increased compared to normal wood, with the development of a thick GL. However, even after GL formation, SL thickness continues to increase during the growing season. GL thickening was observed to be faster than SL thickening. The development of the unlignified GL is proposed to be a low cost, efficient strategy for a fast generation of tensile stress in broadleaved trees. © 2015 International Association of Wood Anatomists.  
  Address INRA, UR588 Amélioration, Génétique et Physiologie ForestièresOrléans, France  
  Corporate Author Thesis  
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  Notes Export Date: 17 April 2015 Approved no  
  Call Number EcoFoG @ webmaster @ Serial 596  
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Author (up) Agrawal, Anurag A. ; Boroczky, Katalin ; Haribal, Meena ; Hastings, Amy P. ; White, Ronald, A. ; Jiang, Ren-Wang ; Duplais, Christophe doi  openurl
  Title Cardenolides, toxicity, and the costs of sequestration in the coevolutionary interaction between monarchs and milkweeds Type Journal Article
  Year 2021 Publication PNAS Abbreviated Journal  
  Volume 118 Issue 16 Pages e2024463118  
  Keywords  
  Abstract For highly specialized insect herbivores, plant chemical defenses are often co-opted as cues for oviposition and sequestration. In such interactions, can plants evolve novel defenses, pushing herbivores to trade off benefits of specialization with costs of coping with toxins? We tested how variation in milkweed toxins (cardenolides) impacted monarch butterfly ( Danaus plexippus ) growth, sequestration, and oviposition when consuming tropical milkweed ( Asclepias curassavica ), one of two critical host plants worldwide. The most abundant leaf toxin, highly apolar and thiazolidine ring–containing voruscharin, accounted for 40% of leaf cardenolides, negatively predicted caterpillar growth, and was not sequestered. Using whole plants and purified voruscharin, we show that monarch caterpillars convert voruscharin to calotropin and calactin in vivo, imposing a burden on growth. As shown by in vitro experiments, this conversion is facilitated by temperature and alkaline pH. We next employed toxin-target site experiments with isolated cardenolides and the monarch’s neural Na + /K + -ATPase, revealing that voruscharin is highly inhibitory compared with several standards and sequestered cardenolides. The monarch’s typical >50-fold enhanced resistance to cardenolides compared with sensitive animals was absent for voruscharin, suggesting highly specific plant defense. Finally, oviposition was greatest on intermediate cardenolide plants, supporting the notion of a trade-off between benefits and costs of sequestration for this highly specialized herbivore. There is apparently ample opportunity for continued coevolution between monarchs and milkweeds, although the diffuse nature of the interaction, due to migration and interaction with multiple milkweeds, may limit the ability of monarchs to counteradapt.  
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  Publisher National Academy of Sciences Place of Publication Editor  
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  Notes Approved no  
  Call Number EcoFoG @ webmaster @ Serial 1014  
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Author (up) Aguilos, M.; Hérault, B.; Burban, B.; Wagner, F.; Bonal, D. url  doi
openurl 
  Title What drives long-term variations in carbon flux and balance in a tropical rainforest in French Guiana? Type Journal Article
  Year 2018 Publication Agricultural and Forest Meteorology Abbreviated Journal  
  Volume 253–254 Issue Pages 114-123  
  Keywords Tropical rainforest; Nee; Gpp; Ecosystem respiration; Radiation; Drought  
  Abstract A thorough understanding of how tropical forests respond to climate is important to improve ecosystem process models and to reduce uncertainties in current and future global carbon balance calculations. The Amazon rainforest, a major contributor to the global carbon cycle, is subject to strong intra- and interannual variations in climate conditions. Understanding their effect on carbon fluxes between the ecosystem and the atmosphere and on the resulting carbon balance is still incomplete. We examined the long-term (over a 12-year period; 2004–2015) variations in gross primary productivity (GPP), ecosystem respiration (RE) and net ecosystem exchange (NEE) in a tropical rainforest in French Guiana and identified key climatic drivers influencing the changes. The study period was characterized by strong differences in climatic conditions among years, particularly differences in the intensity of the dry and wet seasons, as well as differences in annual carbon fluxes and balance. Annual average GPP varied from 3384.9?g?C?m-2?yr?1 (95% CI [3320.7, 3445.9]) to 4061.2?g?C?m-2?yr?1 (95% CI [3980.1, 4145.0]). RE varied even more than GPP, with a difference of 933.1?C?m-2?yr?1 between the minimum (3020.6?g?C?m-2?yr?1; 95% CI [2889.4, 3051.3]) and maximum (3953.7?g?C?m-2?yr?1; 95% CI [3887.6, 4019.6]) values. Although NEE showed large interannual variability (nine-fold), from ?65.6?g?C?m-2?yr?1 (95% CI [?4.4, ?126.0]) to ?590.5?g?C?m-2 yr?1 (95% CI [?532.3, ?651.6]), the forest remained a carbon sink over the 12-year period. A combination of global radiation (Rg), relative extractable water (REW) and soil temperature (Ts) explained 51% of the daily variations for GPP, 30% for RE and 39% for NEE. Global radiation was always the best predictor of these variations, but soil water content and temperature did also influence carbon fluxes and balance. Seasonally, Rg was the major controlling factor for GPP, RE and NEE during the wet season. During the dry season, variations in carbon fluxes and balance were poorly explained by climate factors. Yet, REW was the key driver of variations in NEE during the dry season. This study highlights that, over the long-term, carbon fluxes and balance in such tropical rainforest ecosystems are largely controlled by both radiation and water limitation. Even though variations in Rg have a greater impact on these fluxes, water limitation during seasonal droughts is enough to reduce ecosystem productivity, respiration and carbon uptake. The reduced precipitation expected in tropical rainforest areas under future climatic conditions will therefore strongly influence carbon fluxes and carbon uptake. This study also highlights the importance for land surface or dynamic global vegetation models to consider the main drivers of carbon fluxes and balance separately for dry and wet seasons.  
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  ISSN 0168-1923 ISBN Medium  
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  Notes Approved no  
  Call Number EcoFoG @ webmaster @ Serial 792  
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Author (up) Aguilos, M.; Stahl, C.; Burban, B.; Hérault, B.; Courtois, E.; Coste, S.; Wagner, F.; Ziegler, C.; Takagi, K.; Bonal, D. pdf  url
doi  openurl
  Title Interannual and seasonal variations in ecosystem transpiration and water use efficiency in a tropical rainforest Type Journal Article
  Year 2018 Publication Forests Abbreviated Journal Forests  
  Volume 10 Issue 1 Pages  
  Keywords Drought; Evapotranspiration; Radiation; Tropical rainforest; Water use efficiency; Atmospheric radiation; Carbon dioxide; Climate change; Drought; Efficiency; Evapotranspiration; Forestry; Heat radiation; Radiation effects; Soil moisture; Tropics; Water supply; Climate condition; Drought conditions; Interannual variability; Mechanistic models; Seasonal variation; Tropical ecosystems; Tropical rain forest; Water use efficiency; Ecosystems  
  Abstract Warmer and drier climates over Amazonia have been predicted for the next century with expected changes in regional water and carbon cycles. We examined the impact of interannual and seasonal variations in climate conditions on ecosystem-level evapotranspiration (ET) and water use efficiency (WUE) to determine key climatic drivers and anticipate the response of these ecosystems to climate change. We used daily climate and eddyflux data recorded at the Guyaflux site in French Guiana from 2004 to 2014. ET and WUE exhibited weak interannual variability. The main climatic driver of ET and WUE was global radiation (Rg), but relative extractable water (REW) and soil temperature (Ts) did also contribute. At the seasonal scale, ET and WUE showed a modal pattern driven by Rg, with maximum values for ET in July and August and for WUE at the beginning of the year. By removing radiation effects during water depleted periods, we showed that soil water stress strongly reduced ET. In contrast, drought conditions enhanced radiation-normalized WUE in almost all the years, suggesting that the lack of soil water had a more severe effect on ecosystem evapotranspiration than on photosynthesis. Our results are of major concern for tropical ecosystem modeling because they suggest that under future climate conditions, tropical forest ecosystems will be able to simultaneously adjust CO2 and H2O fluxes. Yet, for tropical forests under future conditions, the direction of change in WUE at the ecosystem scale is hard to predict, since the impact of radiation on WUE is counterbalanced by adjustments to soil water limitations. Developing mechanistic models that fully integrate the processes associated with CO2 and H2O flux control should help researchers understand and simulate future functional adjustments in these ecosystems.  
  Address Hokkaido University, Sapporo, 060-0808, Japan  
  Corporate Author Thesis  
  Publisher Mdpi Ag Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 19994907 (Issn) ISBN Medium  
  Area Expedition Conference  
  Notes Export Date: 1 February 2019; Correspondence Address: Bonal, D.; Université de Lorraine, AgroParisTech, INRA, UMR SilvaFrance; email: damien.bonal@inra.fr; References: Von Randow, C., Zeri, M., Restrepo-Coupe, N., Muza, M.N., de Gonçalves, L.G.G., Costa, M.H., Araujo, A.C., Saleska, S.R., Interannual variability of carbon and water fluxes in Amazonian forest, Cerrado and pasture sites, as simulated by terrestrial biosphere models (2013) Agric. For. Meteorol, 182-183, pp. 145-155; Duffy, P.B., Brando, P., Asner, G.P., Field, C.B., Projections of future meteorological drought and wet periods in the Amazon (2015) Proc. Natl. Acad. Sci. USA, 112, pp. 13172-13177; Cox, P.M., Betts, R.A., Collins, M., Harris, P.P., Huntingford, C., Jones, C.D., Amazonian forest dieback under climate-carbon cycle projections for the 21st century (2004) Theor. Appl. Climatol, 78, pp. 137-156; Poulter, B., Hattermann, F., Hawkins, E., Zaehle, S., Sitch, S., Restrepo-Coupe, N., Heyder, U., Cramer, W., Robust dynamics of Amazon dieback to climate change with perturbed ecosystem model parameters (2010) Glob. Chang. Biol, 16, pp. 2476-2495; Saleska, S.R., Didan, K., Huete, A.R., Da Rocha, H.R., Amazon forests green-up during 2005 drought (2007) Science, 318, p. 612; Phillips, O.L., Aragão, L.E.O.C., Lewis, S.L., Fisher, J.B., Lloyd, J., López-González, G., Malhi, Y., Quesada, C.A., Drought sensitivity of the amazon rainforest (2009) Science, 323, pp. 1344-1347; Bonal, D., Burban, B., Stahl, C., Wagner, F., Hérault, B., The response of tropical rainforests to drought-Lessons from recent research and future prospects (2016) Ann. For. Sci, 73, pp. 27-44; Wang, K.C., Dickinson, R.E., A review of global terrestrial evapotranspiration: Observation, modeling, climatology, and climatic variability (2012) Rev. Geophys, p. 50; Fisher, R.A., Williams, M., da Costa, A.L., Malhi, Y., da Costa, R.F., Almeida, S., Meir, P., The response of an Eastern Amazonian rain forest to drought stress: Results and modelling analyses from a throughfall exclusion experiment (2007) Glob. Chang. Biol, 13, pp. 2361-2378; Costa, M.H., Biajoli, M.C., Sanches, L., Malhado, A.C.M., Hutyra, L.R., Da Rocha, H.R., Aguiar, R.G., De Araújo, A.C., Atmospheric versus vegetation controls of Amazonian tropical rain forest evapotranspiration: Are the wet and seasonally dry rain forests any different? (2010) J. Geophys. Res. Biogeosci, 115, pp. 1-9; Carswell, F.E., Costa, A.L., Palheta, M., Malhi, Y., Meir, P., Costa, J.D.P.R., Ruivo, M.D.L., Clement, R.J., Seasonality in CO2 and H2O flux at an eastern Amazonian rain forest (2002) J. Geophys. Res. D Atmos, 107, p. 8076; Hasler, N., Avissar, R., What controls evapotranspiration in the Amazon basin? (2007) J. Hydrometeorol, 8, pp. 380-395; Da Rocha, H.R., Manzi, A.O., Cabral, O.M., Miller, S.D., Goulden, M.L., Saleska, S.R., Coupe, N.R., Artaxo, R., Patterns of water and heat flux across a biome gradient from tropical forest to savanna in brazil (2009) J. Geophys. Res. Biogeosci, p. 114; Kim, Y., Knox, R.G., Longo, M., Medvigy, D., Hutyra, L.R., Pyle, E.H., Wofsy, S.C., Moorcroft, P.R., Seasonal carbon dynamics and water fluxes in an Amazon rainforest (2012) Glob. Chang. Biol, 18, pp. 1322-1334; Maeda, E.E., Ma, X., Wagner, F.H., Kim, H., Oki, T., Eamus, D., Huete, A., Evapotranspiration seasonality across the Amazon Basin (2017) Earth Syst. Dyn, 8, pp. 439-454; Farquhar, G.D., Ehleringer, J.R., Hubick, K.T., Carbon isotope discrimination and photosynthesis (1989) Ann. Rev. Plant Physiol, 40, pp. 503-537; Hutyra, L.R., Munger, J.W., Saleska, S.R., Gottlieb, E., Daube, B.C., Dunn, A.L., Amaral, D.F., Wofsy, S.C., Seasonal controls on the exchange of carbon and water in an Amazonian rain forest (2007) J. Geophys. Res. Biogeosci; Negrón Juárez, R.I., Hodnett, M.G., Fu, R., Gouden, M.L., von Randow, C., Control of dry season evapotranspiration over the Amazonian forest as inferred from observation at a Southern Amazon forest site (2007) J. Clim, 20, pp. 2827-2839; Fisher, J.B., Malhi, Y., Bonal, D., Da Rocha, H.R., De Araújo, A.C., Gamo, M., Goulden, M.L., Kondo, H., The land-atmosphere water flux in the tropics (2009) Glob. Chang. Biol; Christoffersen, B.O., Restrepo-Coupe, N., Arain, M.A., Baker, I.T., Cestaro, B.P., Ciais, P., Fisher, J.B., Gulden, L., Mechanisms of water supply and vegetation demand govern the seasonality and magnitude of evapotranspiration in Amazonia and Cerrado (2014) Agric. For. Meteorol, 191, pp. 33-50; Da Costa, A.C.L., Rowland, L., Oliveira, R.S., Oliveira, A.A.R., Binks, O.J., Salmon, Y., Vasconcelos, S.S., Poyatos, R., Stand dynamics modulate water cycling and mortality risk in droughted tropical forest (2018) Glob. Chang. Biol; Huang, M., Piao, S., Sun, Y., Ciais, P., Cheng, L., Mao, J., Poulter, B., Wang, Y., Change in terrestrial ecosystem water-use efficiency over the last three decades (2015) Glob. Chang. Biol; Brienen, R.J.W., Wanek, W., Hietz, P., Stable carbon isotopes in tree rings indicate improved water use efficiency and drought responses of a tropical dry forest tree species (2011) Trees, 25, pp. 103-113; Yu, G., Song, X., Wang, Q., Liu, Y., Guan, D., Yan, J., Sun, X., Wen, X., Water-use efficiency of forest ecosystems in eastern China and its relations to climatic variables (2008) New Phytol, 177, pp. 927-937; Aguilos, M., Hérault, B., Burban, B., Wagner, F., Bonal, D., What drives long-term variations in carbon flux and balance in a tropical rainforest in French Guiana? Agric (2018) For. Meteorol, pp. 253-254; Bonal, D., Bosc, A., Ponton, S., Goret, J.Y., Burban, B.T., Gross, P., Bonnefond, J.M., Epron, D., Impact of severe dry season on net ecosystem exchange in the Neotropical rainforest of French Guiana (2008) Glob. Chang. Biol; Aubinet, M., Grelle, A., Ibrom, A., Rannik, U., Moncrieff, J.B., Foken, T., Kowalski, A.S., Bernhofer, C., Estimates of the annual net carbon and water exchange of forests: The Euroflux methodology (2000) Adv. Ecol. Res, 30, pp. 113-175; Wagner, F., Hérault, B., Stahl, C., Bonal, D., Rossi, V., Modeling water availability for trees in tropical forests (2011) Agric. For. Meteorol, 151, pp. 1202-1213; Kuglitsch, F.G., Reichstein, M., Beer, C., Carrara, A., Ceulemans, R., Granier, A., Janssens, I.A., Loustau, D., Characterisation of ecosystem water-use efficiency of european forests from eddy covariance measurements (2008) Biogeosci. Discuss, 5, pp. 4481-4519; Dekker, S.C., Groenendijk, M., Booth, B.B.B., Huntingford, C., Cox, P.M., Spatial and temporal variations in plant water-use efficiency inferred from tree-ring, eddy covariance and atmospheric observations (2016) Earth Syst. Dyn, 7, pp. 525-533; Yang, Y., Guan, H., Batelaan, O., McVicar, T.R., Long, D., Piao, S., Liang, W., Simmons, C.T., Contrasting responses of water use efficiency to drought across global terrestrial ecosystems (2016) Sci. Rep, 6, p. 23284; Granier, A., Bréda, N., Biron, P., Villette, S., A lumped water balance model to evaluate duration and intensity of drought constraints in forest stands (1999) Ecol. Model, 116, pp. 269-283; Kume, T., Takizawa, H., Yoshifuji, N., Tanaka, K., Tantasirin, C., Tanaka, N., Suzuki, M., Impact of soil drought on sap flow and water status of evergreen trees in a tropical monsoon forest in northern Thailand (2007) For. Ecol. Manag, 238, pp. 220-230; Xiao, J., Sun, G., Chen, J., Chen, H., Chen, S., Dong, G., Gao, S., Han, S., Carbon fluxes, evapotranspiration, and water use efficiency of terrestrial ecosystems in China (2013) Agric. For. Meteorol; Boese, S., Jung, M., Carvalhais, N., Reichstein, M., The importance of radiation for semi-empirical water-use efficiency models (2017) Biogeosciences, 14, pp. 3015-3026; Bonal, D., Ponton, S., Le Thiec, D., Richard, B., Ningre, N., Hérault, B., Ogée, J., Sabatier, D., Leaf functional response to increasing atmospheric CO2 concentrations over the last century in two northern Amazonian tree species: An historical δ13C and δ18O approach using herbarium samples (2011) Plant Cell Environ, 34, pp. 1332-1344; Wagner, F., Rossi, V., Stahl, C., Bonal, D., Hérault, B., Water availability is the main climate driver of neotropical tree growth (2012) PLoS ONE, 7; Van der Molen, M.K., Dolman, A.J., Ciais, P., Eglin, T., Gobron, N., Law, B.E., Meir, P., Reichstein, M., Drought and ecosystem carbon cycling (2011) Agric. For. Meteorol, 151, pp. 765-773; Allen, C.D., Macalady, A.K., Chenchouni, H., Bachelet, D., McDowell, N., Vennetier, M., Kitzberger, T., Hogg, E.H., A global overview of drought and heat-induced tree mortality reveals emerging climate change risks for forests (2010) For. Ecol. Manag, 259, pp. 660-684; Da Rocha, H.R., Goulden, M.L., Miller, S.D., Menton, M.C., Pinto, L.D., De Freitas, H.C., Seasonality of water and heat fluxes over a tropical forest in eastern Amazonia (2004) Ecol. Appl, 14, pp. 22-32; Baldocchi, D., Falge, E., Gu, L., Olson, R., Hollinger, D., Running, S., Anthoni, P., Evans, R., FLUXNET: A New tool to study the temporal and spatial variability of ecosystem-scale carbon dioxide, water vapor, and energy flux densities (2001) Bull. Am. Meteorol. Soc, 82, pp. 2415-2434; Stahl, C., Hérault, B., Rossi, V., Burban, B., Bréchet, C., Bonal, D., Depth of soil water uptake by tropical rainforest trees during dry periods: Does tree dimension matter? (2013) Oecologia, 173, pp. 1191-1201; Nepstad, D.C., De Carvalho, C.R., Davidson, E.A., Jipp, P.H., Lefebvre, P.A., Negreiros, G.H., Da Silva, E.D., Vieira, S., The role of deep roots in the hydrological and carbon cycles of Amazonian forests and pastures (1994) Nature; Lee, J.-E., Boyce, K., Impact of the hydraulic capacity of plants on water and carbon fluxes in tropical South America (2010) J. Geophys. Res; Xiao, X., Zhang, Q., Saleska, S., Hutyra, L., De Camargo, P., Wofsy, S., Frolking, S., Moore, B., Satellite-based modeling of gross primary production in a seasonally moist tropical evergreen forest (2005) Remote Sens. Environ, 94, pp. 105-122; Wagner, F.H., Hérault, B., Bonal, D., Stahl, C., Anderson, L.O., Baker, T.R., Becker, G.S., Botosso, P.C., Climate seasonality limits leaf carbon assimilation and wood productivity in tropical forests (2016) Biogeosciences, 13, pp. 2537-2562; Stahl, C., Burban, B., Wagner, F., Goret, J.-Y., Bompy, F., Bonal, D., Influence of Seasonal Variations in Soil Water Availability on Gas Exchange of Tropical Canopy Trees (2013) Biotropica, 45, pp. 155-164; Maréchaux, I., Bonal, D., Bartlett, M.K., Burban, B., Coste, S., Courtois, E.A., Dulormne, M., Mirabel, A., Dry-season decline in tree sapflux is correlated with leaf turgor loss point in a tropical rainforest (2018) Funct. Ecol, 32, pp. 2285-2297; Chaves, M.M., Maroco, J.P., Pereira, J.S., Understanding plant responses to drought-from genes to the whole plant (2003) Funct. Plant Biol, 30, pp. 239-264 Approved no  
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Author (up) Aili, S.R.; Touchard, A.; Escoubas, P.; Padula, M.P.; Orivel, J.; Dejean, A.; Nicholson, G.M. url  openurl
  Title Diversity of peptide toxins from stinging ant venoms Type Journal Article
  Year 2014 Publication Toxicon Abbreviated Journal Toxicon  
  Volume 92 Issue Pages 166-178  
  Keywords Ant venom; Chemotaxonomy; Disulfide linkage; Peptides; Venom biochemistry  
  Abstract Ants (Hymenoptera: Formicidae) represent a taxonomically diverse group of arthropods comprising nearly 13,000 extant species. Sixteen ant subfamilies have individuals that possess a stinger and use their venom for purposes such as a defence against predators, competitors and microbial pathogens, for predation, as well as for social communication. They exhibit a range of activities including antimicrobial, haemolytic, cytolytic, paralytic, insecticidal and pain-producing pharmacologies. While ant venoms are known to be rich in alkaloids and hydrocarbons, ant venoms rich in peptides are becoming more common, yet remain understudied. Recent advances in mass spectrometry techniques have begun to reveal the true complexity of ant venom peptide composition. In the few venoms explored thus far, most peptide toxins appear to occur as small polycationic linear toxins, with antibacterial properties and insecticidal activity. Unlike other venomous animals, a number of ant venoms also contain a range of homodimeric and heterodimeric peptides with one or two interchain disulfide bonds possessing pore-forming, allergenic and paralytic actions. However, ant venoms seem to have only a small number of monomeric disulfide-linked peptides. The present review details the structure and pharmacology of known ant venom peptide toxins and their potential as a source of novel bioinsecticides and therapeutic agents.  
  Address Laboratoire Écologie Fonctionnelle et Environnement, Université de Toulouse, 118 Route de NarbonneToulouse, France  
  Corporate Author Thesis  
  Publisher Elsevier Ltd Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 00410101 (Issn) ISBN Medium  
  Area Expedition Conference  
  Notes Export Date: 2 December 2014; Coden: Toxia; Correspondence Address: Nicholson, G.M.; Neurotoxin Research Group, School of Medical and Molecular Biosciences, University of Technology SydneyAustralia Approved no  
  Call Number EcoFoG @ webmaster @ Serial 568  
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Author (up) Aili, S.R.; Touchard, A.; Hayward, R.; Robinson, S.D.; Pineda, S.S.; Lalagüe, H.; Mrinalini; Vetter, I.; Undheim, E.A.B.; Kini, R.M.; Escoubas, P.; Padula, M.P.; Myers, G.S.A.; Nicholson, G.M. doi  openurl
  Title An integrated proteomic and transcriptomic analysis reveals the venom complexity of the bullet ant Paraponera clavata Type Journal Article
  Year 2020 Publication Toxins Abbreviated Journal Toxins  
  Volume 12 Issue 5 Pages  
  Keywords DRG neurons; Hyaluronidase; Neurotoxins; Paraponeritoxin; Phospholipases; Rp-Hplc; alpha latrotoxin; ant venom; arginine kinase; cathepsin; contig; defensin 2; hyaluronidase; icarapin; metalloproteinase; neurotoxin; novel toxin like protein; phospholipase; phospholipase A2; poneratoxin; proteome; serine proteinase; transcriptome; unclassified drug; amino acid sequence; ant; Article; liquid chromatography-mass spectrometry; neurotoxicity; nonhuman; Paraponera clavata; protein expression; proteomics; sequence database; tandem mass spectrometry; transcriptomics; venom gland  
  Abstract A critical hurdle in ant venom proteomic investigations is the lack of databases to comprehensively and specifically identify the sequence and function of venom proteins and peptides. To resolve this, we used venom gland transcriptomics to generate a sequence database that was used to assign the tandem mass spectrometry (MS) fragmentation spectra of venom peptides and proteins to specific transcripts. This was performed alongside a shotgun liquid chromatography-mass spectrometry (LC-MS/MS) analysis of the venom to confirm that these assigned transcripts were expressed as proteins. Through the combined transcriptomic and proteomic investigation of Paraponera clavata venom, we identified four times the number of proteins previously identified using 2D-PAGE alone. In addition to this, by mining the transcriptomic data, we identified several novel peptide sequences for future pharmacological investigations, some of which conform with inhibitor cysteine knot motifs. These types of peptides have the potential to be developed into pharmaceutical or bioinsecticide peptides. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.  
  Address Faculty of Science, University of Nice, Nice, 06000, France  
  Corporate Author Thesis  
  Publisher Mdpi Ag Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 20726651 (Issn) ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number EcoFoG @ webmaster @ Serial 972  
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Author (up) Aili, S.R.; Touchard, A.; Koh, J.M.S.; Dejean, A.; Orivel, J.; Padula, M.P.; Escoubas, P.; Nicholson, G.M. url  doi
openurl 
  Title Comparisons of Protein and Peptide Complexity in Poneroid and Formicoid Ant Venoms Type Journal Article
  Year 2016 Publication Journal of Proteome Research Abbreviated Journal Journal of Proteome Research  
  Volume 15 Issue 9 Pages 3039-3054  
  Keywords ant venom; Hymenoptera; Lc-Maldi-Tof Ms; mass spectrometry; nanoLC-ESI-QTOF MS/MS; peptidome; proteomic analysis; toxin  
  Abstract Animal venom peptides are currently being developed as novel drugs and bioinsecticides. Because ants use venoms for defense and predation, venomous ants represent an untapped source of potential bioactive toxins. This study compared the protein and peptide components of the poneroid ants Neoponera commutata, Neoponera apicalis, and Odontomachus hastatus and the formicoid ants Ectatomma tuberculatum, Ectatomma brunneum, and Myrmecia gulosa. 1D and 2D PAGE revealed venom proteins in the mass range <10 to >250 kDa. NanoLC-ESI-QTOF MS/MS analysis of tryptic peptides revealed the presence of common venom proteins and also many undescribed proteins. RP-HPLC separation followed by MALDI-TOF MS of the venom peptides also revealed considerable heterogeneity. It was found that the venoms contained between 144 and 1032 peptides with 5-95% of peptides in the ranges 1-4 and 1-8 kDa for poneroid and formicoid ants, respectively. By employing the reducing MALDI matrix 1,5-diaminonapthalene, up to 28 disulfide-bonded peptides were also identified in each of the venoms. In particular, the mass range of peptides from poneroid ants is lower than peptides from other venoms, indicating possible novel structures and pharmacologies. These results indicate that ant venoms represent an enormous, untapped source of novel therapeutic and bioinsecticide leads. © 2016 American Chemical Society.  
  Address VenomeTech, 473 Route des Dolines, Valbonne, France  
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  Notes Export Date: 15 September 2016 Approved no  
  Call Number EcoFoG @ webmaster @ Serial 695  
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Author (up) Aili, S.R.; Touchard, A.; Petitclerc, F.; Dejean, A.; Orivel, J.; Padula, M.P.; Escoubas, P.; Nicholson, G.M. doi  openurl
  Title Combined Peptidomic and Proteomic Analysis of Electrically Stimulated and Manually Dissected Venom from the South American Bullet Ant Paraponera clavata Type Journal Article
  Year 2017 Publication Journal of Proteome Research Abbreviated Journal J. Proteome Res.  
  Volume 16 Issue 3 Pages 1339-1351  
  Keywords  
  Abstract Ants have evolved venoms rich in peptides and proteins used for predation, defense, and communication. However, they remain extremely understudied due to the minimal amount of venom secreted by each ant. The present study investigated the differences in the proteome and peptidome of the venom from the bullet ant, Paraponera clavata. Venom samples were collected from a single colony either by manual venom gland dissection or by electrical stimulation and were compared using proteomic methods. Venom proteins were separated by 2D-PAGE and identified by nanoLC-ESI-QTOF MS/MS. Venom peptides were initially separated using C18 reversed-phase high-performance liquid chromatography, then analyzed by MALDI-TOF MS. The proteomic analysis revealed numerous proteins that could be assigned a biological function (total 94), mainly as toxins, or roles in cell regulation and transport. This investigation found that ca. 73% of the proteins were common to venoms collected by the two methods. The peptidomic analysis revealed a large number of peptides (total 309) but with <20% shared by the two collection methods. There was also a marked difference between venoms obtained by venom gland dissection from different ant colonies. These findings demonstrate the rich composition and variability of P. clavata venom.  
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  Publisher American Chemical Society Place of Publication Editor  
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  ISSN 1535-3893 ISBN Medium  
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  Call Number EcoFoG @ webmaster @ Serial 746  
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Author (up) Aimene, Y.; Dorville, R.; Omrane, A. url  openurl
  Title Optimal control for trees trunk diameter estimation in rain forest ecology Type Journal Article
  Year 2013 Publication Applied Mathematical Sciences Abbreviated Journal  
  Volume 7 Issue 17-20 Pages 807-816  
  Keywords Missing data; Optimal control; Tree trunk diameter  
  Abstract We study the optimal control question for an important mechanical problem related to tree trunk diameter variation in tropical forest ecology where some data are missing. Within a cost function, the control problem is formulated with a mechanical model that requires boundary conditions tosolve all equations. We give a characterization of the optimal measurement function for the tree trunk problem.  
  Address Laboratoire CEREGMIA EA 2440, Université Antilles-Guyane, I.E.S.G Campus de Trou-Biran, Route de Baduel, 97337 Cayenne, French Guiana  
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  Notes Export Date: 21 February 2013; Source: Scopus Approved no  
  Call Number EcoFoG @ webmaster @ Serial 469  
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Author (up) Aimene, Y.; Vidal-Salle, E.; Hagege, B.; Sidoroff, F.; Boisse, P. openurl 
  Title A Hyperelastic Approach for Composite Reinforcement Large Deformation Analysis Type Journal Article
  Year 2010 Publication Journal of Composite Materials Abbreviated Journal J. Compos Mater.  
  Volume 44 Issue 1 Pages 5-26  
  Keywords hyperelasticity; woven reinforcements; forming; fabric mechanical behavior; finite element  
  Abstract A hyperelastic constitutive model is developed for textile composite reinforcement at large strain. A potential is proposed, which is the addition of two tension and one shear energies. The proposed potential is a function of the right Cauchy Green and structural tensor invariants whose choice corresponds to textile composite reinforcement mechanical behavior which exhibits weak elongations in the fiber directions and large angular variations in the fabric plane. The model is implemented in a Vumat user routine of ABAQUS/Explicit. Some elementary tests are performed in order to identify the model and verify its validity. It is then used to simulate the hemispherical punch forming of balanced and unbalanced fabrics. A correct agreement is obtained with experimental forming processes.  
  Address [Aimene, Y.; Vidal-Salle, E.; Boisse, P.] Univ Lyon, INSA Lyon, LaMCoS, F-69621 Villeurbanne, France, Email: philippe.boisse@insa-lyon.fr  
  Corporate Author Thesis  
  Publisher SAGE PUBLICATIONS LTD Place of Publication Editor  
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  ISSN 0021-9983 ISBN Medium  
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  Notes ISI:000273509100001 Approved no  
  Call Number EcoFoG @ eric.marcon @ Serial 84  
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