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Author Zinger, L.; Donald, J.; Brosse, S.; Gonzalez, M.A.; Iribar, A.; Leroy, C.; Murienne, J.; Orivel, J.; Schimann, H.; Taberlet, P.; Lopes, C.M. doi  openurl
  Title Advances and prospects of environmental DNA in neotropical rainforests Type Journal Article
  Year 2020 Publication Advances in Ecological Research Abbreviated Journal Adv. Ecol. Res.  
  Volume 62 Issue Pages 331-373  
  Keywords Biomonitoring; Conservation biology; DNA metabarcoding; eDNA; Environmental genomics; Neotropics; Rainforests  
  Abstract The rainforests of the Neotropics shelter a vast diversity of plant, animal and microscopic species that provide critical ecosystem goods and services for both local and worldwide populations. These environments face a major crisis due to increased deforestation, pollution, and climate change, emphasizing the need for more effective conservation efforts. The adequate monitoring of these ecosystems has proven a complex and time consuming endeavour, which depends on ever dwindling taxonomic expertise. To date, many species remain undiscovered, let alone described, with otherwise limited information regarding known species population distributions and densities. Overcoming these knowledge shortfalls and practical limitations is becoming increasingly possible through techniques based on environmental DNA (eDNA), i.e., DNA that can be obtained from environmental samples (e.g. tissues, soil, sediment, water, etc.). When coupled with high-throughput sequencing, these techniques now enable realistic, cost-effective, and standardisable biodiversity assessments. This opens up enormous opportunities for advancing our understanding of complex and species-rich tropical communities, but also in facilitating large-scale biomonitoring programs in the neotropics. In this review, we provide a brief introduction to eDNA methods, and an overview of their current and potential uses in both terrestrial and aquatic ecosystems of neotropical rainforests. We also discuss the limits and challenges of these methods for our understanding and monitoring of biodiversity, as well as future research and applied perspectives of these techniques in neotropical rainforests, and beyond. © 2020 Elsevier Ltd  
  Address Departamento de Zoologia, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, SP, Brazil  
  Corporate Author Thesis  
  Publisher Academic Press Inc. Place of Publication Editor (down) Dumbrell A.J.; Turner E.C.; Fayle T.M.  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title Advances in Ecological Research  
  Series Volume 62 Series Issue Edition  
  ISSN 00652504 (Issn); 9780128211342 (Isbn) ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number EcoFoG @ webmaster @ Serial 995  
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