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Author (up) Zinger, L.; Chave, J.; Coissac, E.; Iribar, A.; Louisanna, E.; Manzi, S.; Schilling, V.; Schimann, H.; Sommeria-Klein, G.; Taberlet, P. url  openurl
  Title Extracellular DNA extraction is a fast, cheap and reliable alternative for multi-taxa surveys based on soil DNA Type Journal Article
  Year 2016 Publication Soil Biology and Biochemistry Abbreviated Journal Soil Biology and Biochemistry  
  Volume 96 Issue Pages 16-19  
  Keywords DNA extraction protocol; DNA metabarcoding; Multi-taxa biodiversity; Tropical forest  
  Abstract DNA metabarcoding on soil samples is increasingly used for large-scale and multi-taxa biodiversity studies. However, DNA extraction may be a major bottleneck for such wide uses. It should be cost/time effective and allow dealing with large sample volumes so as to maximise the representativeness of both micro- and macro-organisms diversity. Here, we compared the performances of a fast and cheap extracellular DNA extraction protocol with a total DNA extraction method in retrieving bacterial, eukaryotic and plant diversity from tropical soil samples of ca. 10 g. The total DNA extraction protocol yielded more high-quality DNA. Yet, the extracellular DNA protocol provided similar diversity assessments although it presented some differences in clades relative abundance and undersampling biases. We argue that extracellular DNA is a good compromise between cost, labor, and accuracy for high-throughput DNA metabarcoding studies of soil biodiversity. © 2016 Elsevier Ltd.  
  Address INRA UMR ECOFOG, Kourou, French Guiana  
  Corporate Author Thesis  
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  Area Expedition Conference  
  Notes Export Date: 17 February 2016 Approved no  
  Call Number EcoFoG @ webmaster @ Serial 663  
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