Method and Isolation of specific primers of candidate genes to mechano-sensing for five Neotropical tree species
Malia Chevelot
Last modified: 2009-09-07
Abstract
Keywords: population genomics, functional gene, mechanical signals, Fabaceae.
Abstract
Mechanical signals have an impact on plant growth development. Ecosystems like the tropical rainforest, where life-history traits related to biomechanics display a high variability, are a privileged study area to better understand the variability through evolutionary processes. Because, tropical species are not model species, the population-genetic studies have historically relied on anonymous loci. We proposed here a method, which takes advantage of the large amounts of annotated sequences available and previous molecular physiology studies on model species. Thank to this, specific molecular markers of known genes for non-model species could be developed, starting from functional annotation of, and homology with, genes isolated in unrelated species. This method led to the successful isolation of 18 candidate genes, 9 of which were likely to be orthologous. Furthemore, it provided 29 polymorphic sequencing markers in six species.
Abstract
Mechanical signals have an impact on plant growth development. Ecosystems like the tropical rainforest, where life-history traits related to biomechanics display a high variability, are a privileged study area to better understand the variability through evolutionary processes. Because, tropical species are not model species, the population-genetic studies have historically relied on anonymous loci. We proposed here a method, which takes advantage of the large amounts of annotated sequences available and previous molecular physiology studies on model species. Thank to this, specific molecular markers of known genes for non-model species could be developed, starting from functional annotation of, and homology with, genes isolated in unrelated species. This method led to the successful isolation of 18 candidate genes, 9 of which were likely to be orthologous. Furthemore, it provided 29 polymorphic sequencing markers in six species.