UMR EcoFoG, 6th Plant Biomechanics Conference

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Method and Isolation of specific primers of candidate genes to mechano-sensing for five Neotropical tree species

Malia Chevelot

Last modified: 2009-09-07

Abstract


Keywords: population genomics, functional gene, mechanical signals, Fabaceae.

Abstract
Mechanical signals have an impact on plant growth development. Ecosystems like the tropical rainforest, where life-history traits related to biomechanics display a high variability, are a privileged study area to better understand the variability through evolutionary processes. Because, tropical species are not model species, the population-genetic studies have historically relied on anonymous loci. We proposed here a method, which takes advantage of the large amounts of annotated sequences available and previous molecular physiology studies on model species. Thank to this, specific molecular markers of known genes for non-model species could be developed, starting from functional annotation of, and homology with, genes isolated in unrelated species. This method led to the successful isolation of 18 candidate genes, 9 of which were likely to be orthologous. Furthemore, it provided 29 polymorphic sequencing markers in six species.